Monoclonal anti EGFR (GFR1195)

BNUB1195-0
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Catalog #: BNUB1195
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Product Description

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Tungekar MF et. al. Journal of Clinical Pathology. 51: 583-587 (1998). |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

EGFR (GFR1195)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

GFR1195

Molecular weight

~170 kDa (wild type) and ~145 kDa (vIII variant)

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Entrez gene ID

1956

SwissProt

P00533

Unigene

488293

Immunogen

Recombinant human EGFR protein

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell surface

Application notes

Digest formalin-fixed tissues with Protease Sigma Cat. #P-6911 at 1mg/mL PBS, pH 7.4 for 10 min at 37C, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 2-4 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user

Positive control

A431 cells. Placenta, Breast, Colon or Bladder cancer

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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