Monoclonal anti GFAP (ASTRO/789)

BNUB0789-0
BNUB0789-1BNUB0789-2
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Catalog #: BNUB0789
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Product Description

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

McLendon, R.E. and Bigner, D.D. 1994. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 4: 221-228. | Eng, L.F. and Ghirnikar, R.S. 1994. GFAP and astrogliosis. Brain Pathol. 4: 229-237. |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

GFAP (ASTRO/789)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

ASTRO/789

Molecular weight

~50 kDa

Synonyms

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Entrez gene ID

2670

SwissProt

P14136

Unigene

514227

Immunogen

Recombinant GFAP protein

Species reactivity

Chicken, Cow, Human, Mouse, Pig, Rabbit, Rat

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.25-0.5 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

Brain or Astrocytoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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