Monoclonal anti HCG alpha (HCGa/53)

BNUB0053-0
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Catalog #: BNUB0053
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Product Description

This MAb reacts with a protein of ~13kDa, identified as α sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the ? and ? subunits. The ? subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

McDonald EA et. al. Endocrinology 150:4358-65 (2009).

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

HCG-alpha (HCGa/53)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

HCGa/53

Molecular weight

~13 kDa

Synonyms
Entrez gene ID

1081

SwissProt

P01215

Unigene

119689

Immunogen

Recombinant hCG alpha protein

Species reactivity

Human

Applications

Immunohistology (formalin)

Cellular localization

Cytoplasmic, Secreted

Application notes

Immunohistology formalin-fixed 1-2 ug/mL for 30 minutes at RT, No special pre-treatment is required for staining of formalin-fixed tissues. Note that HIER is NOT recommended for this antibody., Optimal dilution for a specific application should be determined by user

Positive control

JAR or TT Cells. Placenta

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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