Monoclonal anti Hepatocyte Specific Antigen (HSA133)

BNUB0133-0
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Catalog #: BNUB0133
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Product Description

MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this MAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.

Wennerberg AE et. al. Am J Pathol 1993;143:1050-4. | Ramos-Vara, J.A., et al. Histochem 2002; J. 34: 397-401. | Fan, Z., et al. Mod. Pathol 2003; 16: 137-144, 2003

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Hepatocyte Specific Antigen (HSA133)

Clonality

Monoclonal

Clone

HSA133

Isotype

IgG2b, kappa

Molecular weight

Not Known

Synonyms

Not Known

Entrez gene ID

Not Known

SwissProt

Not Known

Immunogen

SK-H1A9-2 human hepatocellular carcinoma cells

Unigene

Not Known

Species reactivity

Human

Applications

Immunofluorescence

Cellular localization

Cell surface

Application notes

Immunocytochemistry Acetone-fixed cells 0.5-1.0 ug/mL for 30 minutes at RT, Immunofluorescence 0.5-1.0 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Normal liver or hepatocellular carcinoma (HCC)

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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