Monoclonal anti HSP60 (HSPD1/780)

BNUB0780-0
BNUB0780-1BNUB0780-2BNUB0780-3
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Product Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp?s), more appropriately called as stress response proteins (srp?s). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Schlesinger, M.J., et al. 1982. Heat Shock: from Bacteria to Man. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

HSP60 (GROEL/780)

Clonality

Monoclonal

Clone

HSPD1/780

Isotype

IgG1, kappa

Molecular weight

60 kDa

Synonyms
Entrez gene ID

3329

SwissProt

P10809

Unigene

595053

Immunogen

Recombinant human HSPD1 protein

Species reactivity

Chicken, Cow, Dog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Mitochondria in cytoplasm

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.25-0.5 ug/mL

Positive control

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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