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IGF-1 Monoclonal Mouse Antibody (IGF1/1020)

Product Attributes

Antibody number

#1020

Reactivity (target)

IGF-1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

IGF1/1020

Isotype

IgG1, kappa

Molecular weight

~7.6 kDa

Synonyms

Insulin-like Growth Factor IA (IGF-IA); Insulin-like Growth Factor IB (IGF-IB); Mechano Growth Factor (MGF); Somatomedin-C

Human gene symbol

IGF1

Entrez gene ID

3479

SwissProt

P05019

Unigene

160562

Immunogen

Full-length recombinant human IGF-1 protein

Cellular localization

Cytoplasmic, Secreted (extracellular)

Species reactivity

Human, Mouse, Rabbit, Rat

Applications

Immunofluorescence, Flow cytometry

Application notes

Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Supplied As

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified, BSA-free: 1 mg/mL in PBS without azide, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide

Antibody research areas

Cancer, Signal transduction

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 1020, Anti-IGF-1 (IGF1/1020) 

Antibody # prefixConjugationEx/Em (nm)Compatible Lasers (nm)Fluorescence detection channelsSpecial applications/notes
BNC04CF®405S404/431405DAPI (microscopy), Alexa Fluor® 405SIM
BNC05CF®405M408/452405DAPI (microscopy), Pacific Blue™STORM, STED, SIM, 2-Photon, TIRF
BNC06CF®405L395/545405Pacific Orange™Spectral cytometry/imaging
BNC88CF®488A490/515488GFP, FITCSTORM, STED, 2-photon, TIRF
BNC14CF®514516/548488GFP, FITCSpectral cytometry/imaging
BNC43CF®543541/560532RFP, TRITC
BNC55CF®555555/565532, 561RFP, TRITCSTORM
BNC68CF®568562/583532, 561RFP, TRITCSTORM, STED, SIM, TIRF
BNC94CF®594593/614561Texas Red®STORM, STED, 2-photon
BNC40CF®640R642/662633-640Cy®5SIM, TIRF, and FLImP
BNC47CF®647650/665633-640Cy®5STORM
BNC60CF®660C667/685633-640Cy®5Spectral cytometry/imaging, STORM
BNC61CF®660R663/682633-640Cy®5Highly photostable
BNC80CF®680681/698633-640Cy®5.5, IR®700STORM, 3D SMLM, LI-COR® Odssey®
BNC81CF®680R680/701633-640Cy®5.5, IR®7002-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC00CF®700695/720633-640Alexa Fluor® 700Spectral cytometry/imaging
BNC70CF®770770/797785, 808IR®800LI-COR® Odyssey®
BNCRR-PE (PE)496, 546, 565/578488-561PE
BNCAAPC650/660594-633APC
BNCPPerCP482/677488-561PerCP, PE-Cy®5
BNCBBiotinN/AN/AN/A
BNCAPAlkaline PhosphataseN/AN/AN/A
BNCHHorseradish PeroxidaseN/AN/AN/A
BNUBPurified, with BSA N/AN/AN/A
BNUMPurified, BSA-freeN/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Rotwein p, et. al. (1986) J. Biol. Chem. 261: 4828-4832. | Sandberg-Nordqvist AC, et. al. (1993) Cancer Res. 53: 2475-2478. | Zheng WH, et. al. (2000) J. Neural.Transm. Suppl. 2000: 261-272

 

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