Monoclonal anti IL 6 (Mouse) (MP5 20F3)

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Product Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA Capture, Neut)Abrams J, 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA Capture)Litton M, et al. 1994. J. Immunol Meth. 175:47. (IHC)Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. (In Vivo Capture)Starnes HF Jr, et al. 1990. J. Immunol. 145:4185. (Neut)Riedemann NC, et al. 2003. J. Immunol. 170:503. (Neut)Rochman I, et al. 2005. J. Immunol. 174:4761. (Neut)O'Connell PJ, et al. 2006. Blood 107:1010. (ELISA Capture

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Rat

Reactivity (target)

IL-6 (Mouse) (MP5-20F3)

Clonality

Monoclonal

Clone

MP5-20F3

Isotype

IgG2a, kappa

Molecular weight

21 kDa

Synonyms
Entrez gene ID

16193 (Mouse)

SwissProt

P08505 (Mouse)

Immunogen

Recombinant mouse IL-6 protein

Unigene

1019 (Mouse)

Species reactivity

Mouse

Applications

Neutralization, Flow cytometry, Immunofluorescence

Cellular localization

Cytoplasmic and extracellular (secreted)

Application notes

Does not react with human or rat; others not known, Flow Cytometry (0.5-1 ug/million cells in 0.1 mL), Immunofluorescence 0.5-1 ug/mL, Neutralization Studies order Ab without azide, Optimal dilution for a specific application should be determined

Positive control

Mouse heart

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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