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IL-6 Monoclonal Rat Antibody (MP5-20F3)

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Product Attributes

Antibody number

#0663

Antibody reactivity (target)

IL-6

Antibody type

Primary

Host species

Rat

Clonality

Monoclonal

Clone

MP5-20F3

Isotype

IgG1, kappa

Molecular weight

21 kDa

Synonyms

B cell differentiation factor; B-cell stimulatory factor 2 (BSF-2); CTL differentiation factor (CDF); Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HPGF; HSF; Hybridoma growth factor Interferon beta-2; Hybridoma plasmacytoma growth factor; Interferon beta-2 (IFNB2)

Entrez gene ID

16193 (Mouse)

SwissProt

P08505 (Mouse)

Unigene

1019 (Mouse)

Immunogen

Recombinant mouse IL-6 protein

Antibody target cellular localization

Secreted (extracellular)

Species reactivity

Mouse

Expected antibody applications

Flow (intracellular) (published for clone), Functional studies (published for clone)

Antibody application notes

Flow cytometry: 0.5-1 ug/million cells in 0.1 mL, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Does not react with human or rat; others not known, Neutralization Studies order Ab without azide, Optimal dilution for a specific application should be determined by user

Positive control

Mouse heart

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cytokines, Immunology, Inflammation

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0663, Anti-IL-6 (MP5-20F3) 

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. J Immunol (1990) 145(12): 4185-4191. (functional studies)
  2. J Immunol (2013) 190:703-711. (functional studies; Flow)
 

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