Monoclonal anti Mitochondrial Marker (AE 1)

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$120.00

Catalog #: BNUB0905

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Category: Primary Antibodies.

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Product Description

This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Additional Information

Conjugation	Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP
Size	50 uL, 100 uL, 250 uL, 500 uL
Supplied As	Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA
Antibody type	Primary
Host species	Mouse
Reactivity (target)	Mitochondrial Marker (AE-1)
Clonality	Monoclonal
Clone	AE-1
Isotype	IgG's
Molecular weight	60 kDa
Synonyms	Not Known
Entrez gene ID	Not Known
SwissProt	Not Known
Immunogen	Semi-purified mitochondrial preparation
Unigene	Not Known
Species reactivity	Human
Applications	ELISA, Immunofluorescence, Immunohistology (formalin), Western
Cellular localization	Mitochondria in cytoplasm
Application notes	Does not react with mouse or rat; others not known, ELISA For coating, order Ab without BSA, Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.25-0.5 ug/mL
Positive control	HeLa or HepG2 cells. Hepatic carcinoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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