Monoclonal anti Muscle Specific Actin (MSA/953)

BNUB0953-0
BNUB0953-1BNUB0953-2BNUB0953-3BNUB0953-4BNUB0953-5BNUB0953-6
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Catalog #: BNUB0953
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Product Description

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Barkalow, K. and Hartwig, J.H. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr. Biol. 5: 1000-1002. |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Muscle Specific Actin (MSA/953)

Clonality

Monoclonal

Clone

MSA/953

Isotype

IgG1, kappa

Molecular weight

Multiple

Synonyms

ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1

Entrez gene ID

58, 59 & 70

SwissProt

P62736, P68032 & P68133

Immunogen

Synthetic peptide common among human muscle actins

Unigene

118127, 1288 & 500483

Species reactivity

Human, Rabbit, Rat

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.25-0.5 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1.0 ug/mL

Positive control

Muscle or sarcoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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