Monoclonal anti Myogenin (MGN185 + F5D)

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Catalog #: BNUB1233
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Product Description

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms? tumor.

Wang NP et. al. Am J Pathol 1995, 147:1799-1810. | Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 1996; 19:3

Additional Information


Flow cytometry, Immunofluorescence, Immunohistology (formalin)


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

Myogenin (MGN185 + F5D)




MGN185 + F5D



Molecular weight

34 kDa


bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Entrez gene ID







Human myogenin recombinant protein (MGN185); Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)

Species reactivity

Cat, Human, Mouse, Pig, Rat

Cellular localization


Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology (formalin), Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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