Support & Resources

+1

p21 / WAF1 Monoclonal Mouse Antibody (CIP1/823)

Product Attributes

Antibody number

#0823

Reactivity (target)

p21, WAF1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

CIP1/823

Isotype

IgG2a, kappa

Molecular weight

21 kDa

Synonyms

Activating Fragment 1; CAP20; CDK-interacting protein 1; CDKI; CDKN1; CDKN1A; CIP1; Cyclin-dependent kinase inhibitor 1A (p21; Cip1); DNA Synthesis Inhibitor; MDA6; Melanoma Differentiation Associated Protein 6; p21Cip1/Waf1; PIC1; SDI1; SLC12A9; Wild type p53 activated fragment 1 (WAF1)

Human gene symbol

CDKN1A

Entrez gene ID

1026

SwissProt

P38936

Unigene

370771

Immunogen

Recombinant full-length human CDKN1A protein

Verified antibody applications

IF (verified), IHC (FFPE) (verified), WB (verified)

Antibody target cellular localization

Nucleus

Species reactivity

Human

Antibody application notes

Does not react with mouse or rat, others not known, Immunohistology formalin-fixed 2-4 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Western Blot 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HeLa Cells. Skin, colon, or breast carcinoma.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate buffer formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cell cycle, Tumor suppressors

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
50 uL
100 uL
250 uL
500 uL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0823, Anti-p21 (CIP1/823) 

Antibody # prefixConjugationEx/Em (nm)Compatible Lasers (nm)Fluorescence detection channelsSpecial applications/notes
BNC04CF®405S404/431405DAPI (microscopy), Alexa Fluor® 405SIM
BNC05CF®405M408/452405DAPI (microscopy), Pacific Blue™STORM, STED, SIM, 2-Photon, TIRF
BNC06CF®405L395/545405Pacific Orange™Spectral cytometry/imaging
BNC88CF®488A490/515488GFP, FITCSTORM, STED, 2-photon, TIRF
BNC14CF®514516/548488GFP, FITCSpectral cytometry/imaging
BNC43CF®543541/560532RFP, TRITC
BNC55CF®555555/565532, 561RFP, TRITCSTORM
BNC68CF®568562/583532, 561RFP, TRITCSTORM, STED, SIM, TIRF
BNC94CF®594593/614561Texas Red®STORM, STED, 2-photon
BNC40CF®640R642/662633-640Cy®5SIM, TIRF, and FLImP
BNC47CF®647650/665633-640Cy®5STORM
BNC60CF®660C667/685633-640Cy®5Spectral cytometry/imaging, STORM
BNC61CF®660R663/682633-640Cy®5Highly photostable
BNC80CF®680681/698633-640Cy®5.5, IR®700STORM, 3D SMLM, LI-COR® Odssey®
BNC81CF®680R680/701633-640Cy®5.5, IR®7002-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC00CF®700695/720633-640Alexa Fluor® 700Spectral cytometry/imaging
BNC70CF®770770/797785, 808IR®800LI-COR® Odyssey®
BNCRR-PE (PE)496, 546, 565/578488-561PE
BNCAAPC650/660594-633APC
BNCPPerCP482/677488-561PerCP, PE-Cy®5
BNCBBiotinN/AN/AN/A
BNCAPAlkaline PhosphataseN/AN/AN/A
BNCHHorseradish PeroxidaseN/AN/AN/A
BNUBPurified, with BSA N/AN/AN/A
BNUMPurified, BSA-freeN/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

You may also like…