Monoclonal anti p34 / cdk1 (POH 1)

BNUB0853-0
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Product Description

Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Lukas J et. al. European Journal of Biochemistry, 1992, 207(1):169-76

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

p34 / cdk1 (POH-1)

Clonality

Monoclonal

Clone

POH-1

Isotype

IgG1, kappa

Molecular weight

34 kDa

Synonyms
Entrez gene ID

983

SwissProt

P06493

Immunogen

Purified recombinant human p34cdc2 fusion protein

Unigene

334562

Species reactivity

Cow, Human, Mink, Monkey

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Nuclear and cytoplasmic

Application notes

Does not react with mouse, rat, Xenopus, Drosophila, S. pombe, or S. cerevisiae; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 2-4 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

HeLa or MCF-7 Cells or Tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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