Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

p57 / KIP2 Monoclonal Mouse Antibody (KP10)

Recognizes a protein of 57 kDa, identified as p57Kip2.

Product Attributes

Antibody number

0879

Reactivity (target)

p57, KIP2

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

KP10

Isotype

IgG2b, kappa

Molecular weight

57 kDa

Synonyms

Beckwith Wiedemann syndrome (WBS); BWCR; Cyclin dependent kinase inhibitor 1C (CDKN1C); Cyclin dependent kinase inhibitor p57; KIP2; p57; p57Kip2

Human gene symbol

CDKN1C

Entrez gene ID

1028

SwissProt

P49918

Unigene

106070

Immunogen

Recombinant human p57Kip2 protein

Cellular localization

Nucleus

Species reactivity

Mouse, Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 0.25-0.5 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cell cycle, Tumor suppressors

Conjugation
Size
Catalog #
price
Qty
50 uL
100 uL
250 uL
500 uL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0879, Anti-KIP2 (KP10) The prefix indicates conjugation, followed by the antibody number and size suffix.

Antibody # prefixConjugationEx/EmConcentrationStorage Buffer
BNC04CF®405S404/431 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC05CF®405M408/452 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC88CF®488A490/515 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC43CF®543541/560 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC55CF®555555/565 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC68CF®568562/583 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC94CF®594593/614 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC40CF®640R642/662 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC47CF®647650/665 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC61CF®660R663/682 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC80CF®680681/698 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC81CF®680R680/701 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC70CF®770770/797 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCRR-PE (PE)496, 546, 565/578 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAAPC650/660 nnm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCPPerCP482/677 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCBBiotinN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAPAlkaline PhosphataseN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCHHorseradish PeroxidaseN/A0.1 mg/mL PBS, 0.05% BSA, no azide
BNUBPurified, with BSA N/A0.2 mg/mL PBS, 0.05% BSA, 0.05% azide
BNUMPurified, BSA-freeN/A1 mg/mL PBS, no BSA, no azide

References

Lee, M.-H., et al. 1995. Cloning of p57, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 9: 639-649

 

You may also like…