Monoclonal anti PCNA (PC10)

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Catalog #: BNUB0467
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Product Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9. | Hall PA et al. 1990. J. Pathol. 162(4):285-94. | Landberg G & Roos G. 1991. Cancer Res. 51 (17):4570-4. | Woods AL et al. 1991. Histopathol. 19(1):21-7 | Yu,CC. et al. 1991. Histopathol. 19(1):29-33

Additional Information


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)







IgG2a, kappa

Molecular weight

36 kDa


Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

Entrez gene ID





Rat PCNA/Protein A fusion protein


147433 & 728886

Species reactivity

Chicken, Drosophila, Human, Monkey, Mouse, Pig, Rat, S. cerevisiae), S. pombe, Zebrafish


Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Predominantly nuclear, some cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.25-0.5 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

Tonsil or reactive lymph node

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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