Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

PD1 / PDCD1 / CD279 Monoclonal Mouse Antibody (PDCD1/922)

This antibody recognizes PDCD-1 (programmed cell death-1 protein), also designated CD279 or PD-1, a T-cell surface receptor involved in immunosuppression. Inhibiting the PD-1/PD-L1 pathway is a major target of cancer immunotherapy.

Product Attributes

Antibody number

0922

Reactivity (target)

PD1, CD279, PDCD1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

PDCD1/922

Isotype

IgG1, kappa

Molecular weight

55 kDa

Synonyms

CD279; hPD-1; hSLE1; PD1; PDCD1; Programmed Cell Death Protein 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2

Human gene symbol

PDCD1

Entrez gene ID

5133

SwissProt

Q15116

Unigene

158297

Immunogen

Recombinant full-length human PDCD1 protein

Cellular localization

Cytoplasmic, Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

TY cells or Tonsil.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Apoptosis, Cancer, Cancer immunotherapy/immune checkpoint

Tumor expression

Leukemia/lymphoma

Certificates of Analysis (COA)

Request COA
Conjugation
Size
Catalog #
price
Qty
50 uL
100 uL
250 uL
500 uL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

PD-1 (also known as Programmed Death 1, CD279, and PDCD1) is a negative regulatory T cell surface receptor. PD-1 is a Type I transmembrane protein expressed on the plasma membrane of T-cells. It is a T-cell checkpoint protein, involved in preventing autoimmunity. Because binding of PD-1 by its ligands PD-L1 or PD-L2 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0922, Anti-CD279 (PDCD1/922) The prefix indicates conjugation, followed by the antibody number and size suffix.

Antibody # prefixConjugationEx/EmConcentrationStorage Buffer
BNC04CF®405S404/431 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC05CF®405M408/452 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC88CF®488A490/515 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC43CF®543541/560 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC55CF®555555/565 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC68CF®568562/583 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC94CF®594593/614 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC40CF®640R642/662 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC47CF®647650/665 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC61CF®660R663/682 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC80CF®680681/698 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC81CF®680R680/701 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC70CF®770770/797 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCRR-PE (PE)496, 546, 565/578 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAAPC650/660 nnm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCPPerCP482/677 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCBBiotinN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAPAlkaline PhosphataseN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCHHorseradish PeroxidaseN/A0.1 mg/mL PBS, 0.05% BSA, no azide
BNUBPurified, with BSA N/A0.2 mg/mL PBS, 0.05% BSA, 0.05% azide
BNUMPurified, BSA-freeN/A1 mg/mL PBS, no BSA, no azide

References

Ishida, Y., et al. 1992. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11: 3887-3895. |

 

You may also like…