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Nitric Oxide Generation Kit

Various nitric oxide generators for probing biochemical and physiological functions and for controllable NO generation in solution.

Product Attributes

Storage Conditions

See individual components for storage temperature, Desiccate

CAS number

56329-27-2, 136587-13-8, 16142-27-1, 57564-91-7, 79032-48-7

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Product Description

This kit contains 10 mg each of the following nitric oxide donors: SIN-1 (00221), SNAP (00222), S-nitrosoglutathione (00223), spermine NONOate (00224), and DEA-NONOate (00225).

  • SIN-1, SNAP, and SNOG can generate nitric oxide and superoxides
  • NONOate conjugates can generate a controlled release of nitric oxide in a pH-dependent manner
  • Stimulates cyclic GMP production

Fluorescent calcium indicators and these nitric oxide generators are often used to probe the cellular reactions to cell stress, NO, and trace intracellular calcium. NO concentration is most frequently assessed by measuring nitrite level using the Griess Reagent.

References

  1. J Pharmacol Exp Ther 248, 762 (1989), J Pharmacol Exp Ther 248, 762 (1989)
  2. J. Med. Chem 34, 3242 (1991), DOI: 10.1021/jm00115a013
  3. Br J Pharmacol 107, 745 (1992), DOI: 10.1111/j.1476-5381.1992.tb14517.x
  4. Cancer Res. 53, 564-567 (1993), PMID: 8425188
  5. Nature 364, 626 (1993), DOI: 10.1038/364626a0
  6. PNAS 90, 9813 (1993), DOI: 10.1073/pnas.90.21.9813
  7. Neuropharmacology 33, 915 (1994), DOI: 10.1016/0028-3908(94)90190-2
  8. Am J Physiol, L9 (1994), DOI: 10.1152/ajplung.1994.267.1.L9
  9. Life Sci 54, 1449 (1994), DOI: 10.1016/0024-3205(94)00600-8
  10. Neuron 12, 1235 (1994), DOI: 10.1016/0896-6273(94)90440-5
  11. Biochemistry 34, 7177 (1995), DOI: 10.1021/bi00021a032
  12. Biochem Biophys Res Comm 221, 163 (1996), DOI: 10.1006/bbrc.1996.0563
  13. Meth Enzymol 268, 281 (1996), DOI: 10.1016/S0076-6879(96)68030-6
  14. Chem. Res. Toxicol. 23, 2 (2010), DOI: 10.1021/tx900387k
  15. Bioorg Med Chem Lett. 21, 6515 (2011), DOI: 10.1016/j.bmcl.2011.08.071
 

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    Griess Reagent Kit

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    SNAP

    Generate nitric oxide and form superoxides spontaneously under physiological conditions and is often used to probe the cell stress response and stimulate calcium-independent synaptic vesicle release.

    DEA-NONOate

    Releases nitric oxide spontaneously under physiological conditions. Useful for reliable and controllable NO generation in solution.

    Spermine NONOate

    Releases nitric oxide by spontaneously dissociating in a pH-dependent manner and is useful for reliable and controllable NO generation in solution.

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