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SOX9 / SRY-box 9 Monoclonal Mouse Antibody (SOX9/2398)

Product Attributes

Antibody number

#2398

Reactivity (target)

SOX9, SRY-box 9

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

SOX9/2398

Isotype

IgG1, kappa

Molecular weight

65 kDa

Synonyms

Campomelic Dysplasia Autosomal Sex Reversal (CMD1); SRA1; SRXX2; SRY (sex determining region Y) box 9; SRY related HMG box gene 9; Transcription factor SOX9

Human gene symbol

SOX9

Entrez gene ID

6662

SwissProt

P48436

Unigene

647409

Immunogen

Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary)

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Nucleus

Species reactivity

Human

Antibody application notes

Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

HepG2 Cells. Pancreas

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate buffer formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Validated in protein array

Monospecific

Antibody research areas

Cancer, Developmental biology, Transcription factors

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2398, Anti-SOX9 / SRY-box 9 (SOX9/2398) 

Antibody # prefixConjugationEx/Em (nm)Compatible Lasers (nm)Fluorescence detection channelsSpecial applications/notes
BNC04CF®405S404/431405DAPI (microscopy), Alexa Fluor® 405SIM
BNC05CF®405M408/452405DAPI (microscopy), Pacific Blue™STORM, STED, SIM, 2-Photon, TIRF
BNC06CF®405L395/545405Pacific Orange™Spectral cytometry/imaging
BNC88CF®488A490/515488GFP, FITCSTORM, STED, 2-photon, TIRF
BNC14CF®514516/548488GFP, FITCSpectral cytometry/imaging
BNC43CF®543541/560532RFP, TRITC
BNC55CF®555555/565532, 561RFP, TRITCSTORM
BNC68CF®568562/583532, 561RFP, TRITCSTORM, STED, SIM, TIRF
BNC94CF®594593/614561Texas Red®STORM, STED, 2-photon
BNC40CF®640R642/662633-640Cy®5SIM, TIRF, and FLImP
BNC47CF®647650/665633-640Cy®5STORM
BNC60CF®660C667/685633-640Cy®5Spectral cytometry/imaging, STORM
BNC61CF®660R663/682633-640Cy®5Highly photostable
BNC80CF®680681/698633-640Cy®5.5, IR®700STORM, 3D SMLM, LI-COR® Odssey®
BNC81CF®680R680/701633-640Cy®5.5, IR®7002-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC00CF®700695/720633-640Alexa Fluor® 700Spectral cytometry/imaging
BNC70CF®770770/797785, 808IR®800LI-COR® Odyssey®
BNCRR-PE (PE)496, 546, 565/578488-561PE
BNCAAPC650/660594-633APC
BNCPPerCP482/677488-561PerCP, PE-Cy®5
BNCBBiotinN/AN/AN/A
BNCAPAlkaline PhosphataseN/AN/AN/A
BNCHHorseradish PeroxidaseN/AN/AN/A
BNUBPurified, with BSA N/AN/AN/A
BNUMPurified, BSA-freeN/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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