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WarmStart Enzyme Modification Kit

A simple kit for reversibly modifying non-thermostable enzymes like reverse transcriptase, rendering the enzyme inactive during reaction set-up. Activity is restored by heating to 45°C or higher.

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Product Description

The WarmStart™ Enzyme Modification Kit provides an easy way to introduce temperature control of non-thermostable enzymes like reverse transcriptase. The WarmStart™ Enzyme Modification Kit reversibly modifies lysine residues, rendering the enzyme inactive at room temperature. The protein activity is restored by heating to 45°C or higher.

Features

  • Modify non-thermostable enzymes to eliminate activity at room temperature
  • Modification is reversed by heating to 45°C or higher
  • Full-size kit modifies 0.5 mg of enzyme
  • Trial size kit modifies 0.1 mg of enzyme

Kit Components

  • Reaction Buffer
  • Modifying Reagent
  • Storage Buffer
  • Ultrafiltration Vial, 10K MWCO

Potential enzymes to be modified:

  • Reverse transcriptase
  • Bst DNA polymerase
  • E. coli DNA polymerase I
  • Restriction enzymes
  • Nucleases
  • Proteases

The WarmStart™ Enzyme Modification Kit is similar to our HotStart Polymerase Modification Kit (29054).

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