The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components, CF Dye-labeled Concanavalin A (ConA) and Live-or-Dye™ viability dye. There are 3 different color combinations to choose from (if there is a different color combination that you would prefer, email us at techsupport.biotium.com).
Live-or-Dye™ viability dyes are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry or microscopy. They are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. This dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells.
Concanavalin A (Con A) is a widely used lectin that selectively binds to a-mannopyranosyl and a-glucopyranosy residues, found in the cell wall of yeast and fungi. It is labeled with one of our superior bright, photostable CF™ dyes.
After staining yeast with this kit, both living and dead cells will have cell walls stained with Con A, while only dead cells will also have the cytoplasm stained with the Live-or-Dye™.
Kit Components (for 1000 assays):
- Live-or-Dye™ (solid), 4 vials
- Con A (solid), 5 vials
- anhydrous DMSO, 250 uL