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Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit

A fixable, fluorescent assay to determine yeast viability. Contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

Product Attributes

For live or fixed cells

For live/intact cells

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

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1,000 assays
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Product Description

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

Features

  • Stable and selective dead cell staining
  • Simple, rapid, no-wash staining protocol
  • Tolerates formaldehyde fixation
  • Suitable for flow cytometry and microscopy

Kit Components

  • Live-or-Dye™ 568/583 (solid), 4 vials
  • Thiazole Orange 10 mM in DMSO, 100 uL
  • Anhydrous DMSO, 250 uL

Spectral Properties

  • Live-or-Dye™ 568/583 Ex/Em 568/583 nm
  • Thiazole Orange Ex/Em 512/533 nm

How It Works

Live-or-Dye™ 568/583 is a cell membrane impermeable amine-reactive dye. It is able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. This dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. It is suitable for detection by fluorescence microscopy or flow cytometry.

Thiazole Orange is a green fluorescent dye that localizes to the cytoplasm, with some nuclear accumulation. It is fixable, resulting in diffuse cytoplasmic staining in fixed cells.

After staining yeast with this kit, both living and dead cells will be stained with green fluorescence. Only dead cells will be stained with red fluorescence.

Yeast Live-or-Dye Fixable Live/Dead Staining Kit
S. cerevisiae stained with the Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit: Thiazole Orange to stain all cells (green) and Live-or-Dye™ 568/583 to stain dead cells (red, appears yellow in overlay).
 

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