Support & Resources

+1

Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

Product Attributes

For live or fixed cells

For live/intact cells

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

Size
Catalog #
price
Qty
1,000 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

Live-or-Dye™ 568/583 is a fixable red dye, designed for discrimination between live and dead cells during flow cytometry or microscopy. Live-or-Dye™ 568/583 is a cell membrane impermeable amine-reactive dye. It is able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. This dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells.

Thiazole Orange is a green fluorescent dye that localizes to the cytoplasm, with some nuclear accumulation. It is fixable, resulting in diffuse cytoplasmic staining in fixed cells.

After staining yeast with this kit, both living and dead cells will be stained with green fluorescence (Ex/Em 512/533 nm). Only dead cells will be stained with red fluorescence (Ex/Em 568/583 nm), and may appear yellow when the two images are overlaid.

Kit Components (for 1000 assays):

  • Live-or-Dye™ 568/583 (solid), 4 vials
  • Thiazole Orange 10 mM in DMSO, 100 uL
  • Anhydrous DMSO, 250 uL

References

n/a

 

You may also like…

    Yeast Vitality Staining Kit

    The Yeast Vitality Staining Kit is a fluorogenic assay that allows you to determine whether your yeast cells are metabolically active. The kit contains two fluorescent components, Calcofluor White and ViaVac Red/Green.

    Yeast Viability Staining Kit

    The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components, CF® Dye-labeled Concanavalin A (ConA) and Live-or-Dye® viability dye.

    ViaVac™ Red/Green

    ViaVac™ Red/Green is a fluorescent viability dye for yeast, identical in structure to the dye FUN® 1. ViaVac passively diffuses into cells, showing green cytoplasmic staining.

    Calcofluor White Stain, 5 mM in Water

    Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin, which can be found in the cell walls of fungi, algae, and plants. In budding yeast Calcofluor White stains bud scars more strongly, because they have a higher concentration of chitin.

    Thiazole Orange, 10 mM in DMSO

    Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange stains the yeast nucleus, and is also able to stain bacteria and mammalian cells.

    Concanavalin A (Con A) CF® Dye Conjugates

    This product is concanavalin A (Con A) labeled with our superior fluorescent CF® dyes. Con A is a widely used lectin that selectively binds to a-mannopyranosyl and a-glucopyranosy residues, found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells.