Dyes for Mitochondria & Other Organelles
Nonyl Acridine Orange (NAO)
NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential.
Rhodamine 123
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential.
Tetrabromorhodamine 123, bromide
Tetrabromorhodamine 123 can be used to generate singlet oxygen (1O2) in mitochondrial membranes (1,2). The dye has a quantum yield for singlet oxygen generation of 0.65-0.7 and is particularly toxic to carcinoma cells.
TMRE
TMRE (Tetramethylrhodamine ethyl ester, perchlorate) and TMRM (70017) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.
TMRE, 2 mM in DMSO
TMRE (Tetramethylrhodamine ethyl ester, perchlorate) is used to quantify changes in mitochondrial membrane potential in live cells.
TMRM
TMRM (Tetramethylrhodamine methyl ester, perchlorate) and TMRE are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.