LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.
LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. The LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.
LysoView™ staining of microbes varies between dyes:
- LysoView™ 405 stains bacteria, and also stains yeast, but does not localize to vacuoles.
- LysoView™ 488 stains gram-positive bacteria, but not gram-negative bacteria or yeast.
- LysoView™ 540 and LysoView™ 633 stain yeast vacuoles, but don’t stain bacteria.
- LysoView™ 650 does not stain bacteria or yeast.
See our Cellular Stains Table for more information on how our dyes stain various organisms.
*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution.
Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.
Note: LysoView™650 (70059 and 70059-T) is temporarily unavailable.
Cy3 and Cy5 are registered trademarks of GE Healthcare. LysoTracker is a registered trademark of Thermo Fisher Scientific.
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