LysoView™ Dyes
LysoView™ fluorescent dyes label lysosomes in live cells without a wash step, and are available in multiple colors with options for super-resolution imaging.
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Product Description
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.
- No-wash, fluorescent staining of lysosomes in live cells
- Non-toxic for real-time live-cell imaging
- Lower background than LyosoTracker® dyes
- Dye options for super-resolution imaging
- Blue, green, red, or far-red fluorescence
LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 7), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.
LysoView™ 540 and LysoView™ 633 stain vacuoles in yeast. Staining of bacteria varies between dyes. See our Cellular Stains Table for details.
LysoView™ Dyes
Product | Abs/Em | Detection channel | Catalog number | Size |
---|---|---|---|---|
LysoView™ 405, 1000X in DMSO | 318, 400/464 nm | DAPI | 70066-T | 10 uL |
70066 | 50 uL | |||
LysoView™ 488, 1000X in DMSO | 506/532 nm | GFP, FITC | 70067-T | 10 uL |
70067 | 50 uL | |||
LysoView™ 540, 1000X in DMSO | 541/634 nm (pH ≤5) | Rhodamine, Cy®3 | 70061-T | 10 uL |
70061 | 50 uL | |||
LysoView™ 633 (lyophilized solid) | 634/659 nm (pH ≤5) | Cy®5 | 70058-T | 1 vial* |
70058 | 10 vials* | |||
LysoView™ 650, 1000X in DMSO | 650/675 nm | Cy®5 | 70059-T | 10 uL |
70059 | 50 uL |
Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.
References
1. Mol Ther (2016) 24(12), 2100-2108. doi:10.1038/mt.2016.179. (70058)
2. Carbohydrate Polym (2016) 153, 160-168. doi: 10.1016/j.carbpol.2016.07.080 (70058)
3. Leuk Res (2016) 42, 59-67. doi:10.1016/j.leukres.2016.01.007 (70058)
4. bioRxiv (2019) doi: https://doi.org/10.1101/747014 (70058)
5. Int J Pharm (2019) 565, 391-408. doi:10.1016/j.ijpharm.2019.05.009 (70058)
6. Oncogene (2019) 38, 1432–1447. https://doi.org/10.1038/s41388-018-0507-6 (70058)
7. Light Sci Apps (2020) 9, 11. https://doi.org/10.1038/s41377-020-0249-4 (70067, SIM)
8. Spectrochimica Acta A (2020) 233, 118234.https://doi.org/10.1016/j.saa.2020.118234 (70058)
9. Dyes Pigments (2021) 184, 108771 .https://doi.org/10.1016/j.dyepig.2020.108771 (70058)