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LysoView™ Dyes

LysoView™ fluorescent dyes label lysosomes in live cells without a wash step, and are available in multiple colors with options for super-resolution imaging.

Product Attributes

Cellular localization

Lysosomes

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Colors

Blue, Green, Orange, Far-red

Storage Conditions

Store at -10 to -35 °C, Protect from light

Dye
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Trial size (10 uL)
50 uL
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Set of 10 vials
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Product Description

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.

  • No-wash, fluorescent staining of lysosomes in live cells
  • Non-toxic for real-time live-cell imaging
  • Lower background than LyosoTracker® dyes
  • Dye options for super-resolution imaging
  • Blue, green, red, or far-red fluorescence

LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 7), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.

LysoView™ 540 and LysoView™ 633 stain vacuoles in yeast. Staining of bacteria varies between dyes. See our Cellular Stains Table for details.

LysoView™ Dyes

ProductAbs/EmDetection channelCatalog numberSize
LysoView™ 405,
1000X in DMSO
318, 400/464 nmDAPI70066-T10 uL
7006650 uL
LysoView™ 488,
1000X in DMSO
506/532 nmGFP, FITC70067-T10 uL
7006750 uL
LysoView™ 540,
1000X in DMSO
541/634 nm (pH ≤5)Rhodamine, Cy®370061-T10 uL
7006150 uL
LysoView™ 633
(lyophilized solid)
634/659 nm (pH ≤5)Cy®570058-T1 vial*
7005810 vials*
LysoView™ 650,
1000X in DMSO
650/675 nmCy®570059-T10 uL
7005950 uL
*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution. Cy3 and Cy5 are registered trademarks of GE Healthcare.

Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.

LysoTracker is a registered trademark of Thermo Fisher Scientific.

References

1. Mol Ther (2016) 24(12), 2100-2108. doi:10.1038/mt.2016.179. (70058)
2. Carbohydrate Polym (2016) 153, 160-168. doi: 10.1016/j.carbpol.2016.07.080 (70058)
3. Leuk Res (2016) 42, 59-67. doi:10.1016/j.leukres.2016.01.007 (70058)
4. bioRxiv (2019) doi: https://doi.org/10.1101/747014 (70058)
5. Int J Pharm (2019) 565, 391-408. doi:10.1016/j.ijpharm.2019.05.009 (70058)
6. Oncogene (2019) 38, 1432–1447. https://doi.org/10.1038/s41388-018-0507-6 (70058)
7. Light Sci Apps (2020) 9, 11. https://doi.org/10.1038/s41377-020-0249-4 (70067, SIM)
8. Spectrochimica Acta A Mol Biomol Spectrosc (2020) 233, 118234.https://doi.org/10.1016/j.saa.2020.118234 (70058)

 

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