LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. Far-red LysoView™ 650 is compatible with super-resolution imaging by STED and SIM. Note: LysoView™650 (70059 and 70059-T) is temporarily unavailable.
The storage conditions that a Biotium label recommends are deliberately conservative. Long term storage at low temperatures usually will increase the time that the product will remain within specification, unless specific instructions otherwise are provided (such as “do not refrigerate” or “do not freeze”).
Biotium labels and product information sheets may show a specific temperature for long term storage, or a temperature range that is adequate for long term storage. For all products, the implied range is as follows for the following storage conditions:
For Biotium products where the label indicates room temperature or RT, this implies storage in ambient conditions between 20°C and 30°C.
For Biotium products where the label indicates 4°C, this implies storage in a refrigerator that is between 2°C and 8°C.
For Biotium products where the label indicates -20°C, this implies storage in a freezer that is between -35°C and -5°C.
Ultra Low Temperature Freezer
For Biotium products where the label indicates -70°C, this implies storage in an ultra low-temperature (ULT) freezer that is below -60°C.
Compound solubility is listed on product web pages, product information sheets or product safety and data sheets. Check the product information sheet for specific instructions for preparing stock solutions. Stock solutions are generally prepared at 10X-1000X the final required working concentration, typically in the range of 1-100 mg/mL. For dyes and indicators, we generally prepare a concentrated stock solution at 5-10 mM in the recommended solvent. The dye can then be diluted to the final desired concentration in the buffer or medium used for the application.
> 1000 mg/mL
Very Slightly Soluble
< 0.1 mg/mL
To dissolve lyophilized compounds, simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.
Difficult to dissolve compounds can be heated to 50°C or higher (note: we do not recommend heating coelenterazine solutions), and vortexed or sonicated until completely dissolved. Some chemical compounds are just kinetically slow to dissolve. This can be especially true when the compounds are highly pure and in crystalline form. An alternative and gentler way to dissolve these compounds can be to simply rock the compound/solvent mixture in the dark overnight.
When preparing stock solutions in organic solvents such as DMF, DMSO, or alcohol for use in living cells or organisms, make a concentrated stock so that the final concentration of solvent in the working solution will not be toxic. A general guideline for immortalized cell lines is to keep the final solvent concentration below 1%; certain cell lines, primary cells or experimental organisms may be more sensitive to solvent toxicity. Similarly, for enzymatic reactions, the final concentration of solvent should be kept below 1% to avoid inhibiting enzyme activity.
Compounds dissolved in aqueous solution can be sterilized by filtration if necessary. Generally, concentrated stock solutions of compounds in organic solvents do not require sterilization, but culture medium can be sterilized by filtration after addition of the compound.
Some of our large volume light-sensitive products are packaged in amber bottles or amber glass vials, which are easy to handle and protect the products from light. However, amber micro packaging vials (0.5 mL or 2 mL) make it very difficult to see small quantities of dye when preparing solutions or pipetting. Therefore, we only package photoreactive dyes like PMA or PMAxx™ in amber micro packaging vials. Some of our reactive dyes are packaged in transparent vials, then sealed inside a moisture-resistant foil bag, which also will protect them from light.
Other fluorescent dyes in transparent vials should stored in the dark for long term storage. It’s fine to store dye vials uncovered in a windowless refrigerator or freezer with an automatic light shut-off. If dyes will be stored in a glass-front (deli-style) refrigerator, walk-in refrigerator/freezer, or at room temperature with constant light exposure, they should stored in a non-transparent box (like a white cardboard freezer box), in a closed drawer, in a black plastic bag, or covered with aluminum foil.
Fluorescent dyes generally are not sensitive to brief light exposure while they are being handled on the bench during an experiment. To be on the safe side, we usually loosely cover tube racks with a piece of foil if the dye vials are going to be out on the bench for more than 30 minutes or so. But most dyes are stable enough that even accidentally leaving them on the bench for a day will not affect performance. When handling photoreactive dyes like EMA, PMA, or PMAxx, we take the extra precaution of dimming the lights.
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.
LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining.
The LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.
Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.
LysoView™ staining of microbes varies quite a bit between dyes. LysoView™ 405 stains bacteria, and also stains yeast but not vacuolar. LysoView™ 488 stains gram-positive bacteria, but not gram-negative bacteria or yeast. LysoView™ 540 and LysoView™ 633 stain yeast vacuoles, but don’t stain bacteria. And LysoView™ 650 does not stain bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution.
LysoView™650 (70059 and 70059-T) is temporarily unavailable.
Cy3 and Cy5 are registered trademarks of GE Healthcare. LysoTracker is a registered trademark of Thermo Fisher Scientific.
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MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.
NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging.
CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dye phalloidin conjugates consist of the mushroom toxin phalloidin labeled with our superior bright and photostable CF® fluorescent dyes.