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LysoView™ Dyes

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence, with dye options for super-resolution imaging by SIM and STED.

Product Attributes

Cellular localization

Lysosomes

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Colors

Blue, Green, Orange, Far-red

Dye
Size
Catalog #
price
Qty
Trial size (10 uL)
50 uL
Trial size (1 vial)
Set of 10 vials
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Product Description

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.

LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 6), and LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.

LysoView™ staining of microbes varies between dyes:

  • LysoView™ 405 stains bacteria, and also stains yeast, but does not localize to vacuoles.
  • LysoView™ 488 stains gram-positive bacteria, but not gram-negative bacteria or yeast.
  • LysoView™ 540 and LysoView™ 633 stain yeast vacuoles, but don’t stain bacteria.
  • LysoView™ 650 does not stain bacteria or yeast.

See our Cellular Stains Table for more information on how our dyes stain various organisms.

ProductAbs/EmDetection channelCatalog numberSize
LysoView™ 405,
1000X in DMSO
318, 400/464 nmDAPI70066-T10 uL
7006650 uL
LysoView™ 488,
1000X in DMSO
506/532 nmGFP, FITC70067-T10 uL
7006750 uL
LysoView™ 540,
1000X in DMSO
541/634 nm (pH ≤5)Rhodamine, Cy®370061-T10 uL
7006150 uL
LysoView™ 633
(lyophilized solid)
634/659 nm (pH ≤5)Cy®570058-T1 vial*
7005810 vials*
LysoView™ 650,
1000X in DMSO
650/675 nmCy®570059-T10 uL
7005950 uL
*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution.

Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.

Cy3 and Cy5 are registered trademarks of GE Healthcare. LysoTracker is a registered trademark of Thermo Fisher Scientific.

References

1. Mol Ther. 2016; 24(12):2100-2108. doi:10.1038/mt.2016.179.
2. Carbohydrate Polym. 2016; 153:160-168. doi: 10.1016/j.carbpol.2016.07.080
3. Leuk Res. 2016; 42:59-67. doi:10.1016/j.leukres.2016.01.007
4. Int J Pharm. 2019; 565:391-408. doi:10.1016/j.ijpharm.2019.05.009
5. Oncogene 2019; 38:1432–1447. https://doi.org/10.1038/s41388-018-0507-6
6. Light: Sci & Apps. 2020; 9:11. https://doi.org/10.1038/s41377-020-0249-4

 

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