Support & Resources

+1

LysoView™ Dyes

LysoView™ fluorescent dyes label lysosomes in live cells without a wash step, and are available in multiple colors with options for super-resolution imaging.

Product Attributes

Probe cellular localization

Lysosomes

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Storage Conditions

Store at -10 to -35 °C, Protect from light

Dye
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
Trial size (10 uL)
50 uL
Trial size (1 vial)
Set of 10 vials
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

LysoView™ dyes are useful for staining lysosomes in live cells.

  • No-wash, fluorescent staining of lysosomes in live cells
  • Non-toxic for real-time live-cell imaging
  • Lower background than LysoTracker® dyes
  • Dye options for super-resolution imaging
  • Color choices from blue to far-red, plus a unique NIR dye

LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in low pH organelles, resulting in highly specific staining without the need for a wash step. LysoView™ staining is non-toxic, and can imaged over the course of days, even after dye washout for some dye options (see the Product Information Sheet for more information).

LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 7), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.

LysoView™ 540 and LysoView™ 633 stain vacuoles in yeast. Staining of bacteria varies between dyes. See our Cellular Stains Table for details.

Note: LysoView™ 540 has limited photostability, and may not be suitable for all microscopy applications. LysoView™ 550 is a bright and more photostable choice.

Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.

LysoView™ Dyes

ProductCatalog numberSizeAbs/Em (nm)
(pH ≤ 5)
Detection
channel
Features
LysoView™ 405,
1000X in DMSO
70066-T10 uL318, 400/ 464DAPI, Pacific
Blue™
Blue fluorescent
lysosome stain
7006650 uL
LysoView™ 488,
1000X in DMSO
70067-T10 uL496/526GFP, FITCGreen fluorescent dye
validated in SIM
7006750 uL
LysoView™ 540,
1000X in DMSO
70061-T10 uL540/634TRITC, Cy®3, PEOrange, pH-
sensitive fluorescence**
7006150 uL
LysoView™ 550,
1000X in DMSO
70083-T10 uL542/567TRITC, Cy®3, PEBright & photostable
orange dye
7008350 uL
LysoView™ 594,
1000X in DMSO
70084-T10 uL585/634Texas Red®Bright & photostable
red dye
7008450 uL
LysoView™ 633
(lyophilized solid)
70058-T1 vial*634/657Cy®5, APCFar-red, pH-sensitive fluorescence
7005810 vials*
LysoView™ 640,
1000X in DMSO
70085-T10 uL640/671Cy®5, APCBright & photostable
far-red dye
7008550 uL
LysoView™ 650,
1000X in DMSO
70059-T10 uL650/675Cy®5, APCPhotostable far-red dye
compatible with SIM, STED
7005950 uL
LysoView™ 680,
1000X in DMSO
70086-T10 uL674/711Cy®5.5Unique near-IR
lysosome stain
7008650 uL
*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution.
** LysoView™ 540 has limited photostability and may not be suitable for all microcopy applications.
Cy dye is a registered trademark of GE Healthcare; Pacific Blue is a trademark and Texas Red is a registered trademark of Thermo Fisher Scientific.
LysoTracker is a registered trademark of Thermo Fisher Scientific.

References

  1. Mol Ther (2016) 24(12), 2100-2108. doi:10.1038/mt.2016.179 (LysoView™ 633)
  2. Carbohydrate Polym (2016) 153, 160-168. doi: 10.1016/j.carbpol.2016.07.080 (LysoView™ 633)
  3. Leuk Res (2016) 42, 59-67. doi:10.1016/j.leukres.2016.01.007 (LysoView™ 633)
  4. bioRxiv (2019) doi: https://doi.org/10.1101/747014 (LysoView™ 633)
  5. Int J Pharm (2019) 565, 391-408. doi:10.1016/j.ijpharm.2019.05.009 (LysoView™ 633)
  6. Oncogene (2019) 38, 1432–1447. https://doi.org/10.1038/s41388-018-0507-6 (LysoView™ 633)
  7. Light Sci Apps (2020) 9, 11. https://doi.org/10.1038/s41377-020-0249-4 (LysoView™ 488; SIM)
  8. Spectrochimica Acta A (2020) 233, 118234. https://doi.org/10.1016/j.saa.2020.118234 (LysoView™ 633)
  9. Dyes Pigments (2021) 184, 108771. https://doi.org/10.1016/j.dyepig.2020.108771
    (LysoView™ 633)
  10. Angiogenesis (2021) 24, 657-676. https://doi.org/10.1007/s10456-021-09778-6
    (LysoView™ 633)
  11. Cell Death Dis (2021) 12, 704. https://doi.org/10.1038/s41419-021-03978-0 (LysoView™ 540)
  12. Cell Rep (2021) 36(4) 109434. https://doi.org/10.1016/j.celrep.2021.109434 (LysoView™ 633)
  13. Nanomaterials (2021) 11(7), 1837. https://doi.org/10.3390/nano11071837 (LysoView™ 405)
  14. Sci Adv (2021) 7(21) eabf0573. DOI: 10.1126/sciadv.abf0573 (LysoView™ 405)
  15. Research Square (preprint) (2021). DOI: 10.21203/rs.3.rs-279271/v1 (LysoView™ 488, LipidSpot™ 488; SIM)
 

You may also like…