Apoptosis Assays

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

This product is discontinued and no longer available.

Recombinant human Annexin V expressed from E. coli as a single, non-glycosylated polypeptide chain. The protein is available with no tag or a C-terminal 6X His tag.

Concentrated buffer used for Annexin V staining of cells to monitor apoptosis.

Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

Staurosporine is a potent and cell-permeable inhibitor of a wide variety of protein kinases by competing for the ATP binding site.