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Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

We offer a wide spectrum of CF® Dye colors with a full selection of reactive chemistries, as well as other high quality reactive dyes and biotinylation reagents. This category also includes non-reactive free acid versions of the dyes. Reactive chemistries include:

  • Succinimidyl esters (SE, also known as NHS esters) which react with primary amines such as lysine residues in proteins.
  • Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) which react with aldehydes or ketones to form a stable oxime bond.
  • Maleimides which react with thiol groups to form thioether-coupled products.
  • Dyes with an amine group can be used to conjugate the dye to carboxylic acids groups in biological molecules if the carboxylic acids have been activated using water soluble carbodiimides like EDC.

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    Product

    Mix-n-Stain™ Nanobody Thiol Labeling Kits

    From: $195 Sizes: 5-20 ug labeling, 20-50 ug labelingCatalog #:
    92585
    , 92586, 92587, 92588, 92589, 92590, 92591, 92592, 92593, 92594, 92595, 92596, 92597, - 92598View allHide

    Label 5-50 ug of VHH (also known as Nanobodies®) containing a single free cysteine with Biotium’s bright and photostable CF® Dyes, with no purification after labeling.

     
    Product

    Biotin NTA

    From: $138 Sizes: 5 mgCatalog #: 90074

    Biotin NTA, also known as BNTA (Biotin nitrilotriacetic acid, tripotassium salt) is used to detect histidine-tagged proteins (as little as 0.1 pmol) immobilized on nitrocellulose membranes.

     
    Product

    Biotin-X NTA

    From: $138 Sizes: 5 mgCatalog #: 90056

    Biotin-X NTA (Biotin-X nitrilotriacetic acid, tripotassium salt) is used to detect histidine-tagged proteins (as little as 0.1 pmol) immobilized on nitrocellulose membranes.

     
    Product

    CF® Dye Free Acids

    From: $125 Sizes: 1 mg, 0.25 umolCatalog #:
    92066
    , 92070, 92071, 92159, 98023, 92062, 92063, 92072, 96013, 92116, 92067, - 98004View allHide

    Non-reactive, free acid forms of CF® Dyes that can be used as controls for the corresponding CF® Dye conjugates.

     
    Product

    CF®DI Aminooxy

    From: $245 Sizes: 1 mgCatalog #:
    92177
    , 92178, - 92179View allHide

    CF®DI Aminooxy derivatives are useful for fluorescently labeling aldehyde/ketone groups on biomolecules such as polysaccharides, glycoproteins or antibodies to generate size- and charge-matched conjugates.

     
    Product

    CF® Dye DBCO

    From: $220 Sizes: 1 mgCatalog #:
    96111
    , 96110, 96112, 96113, 96114, 96115, 96116, 96117, 96118, 96119, 96120, 96121, 96122, - 96123View allHide

    CF® Dye with dibenzocyclooctyne (i.e., DBCO, ADIBO) reacts with azide via copper-free click chemistry by strain-promoted azide-alkyne cycloaddition (SPAAC).

     
    Product

    Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits

    From: $287 Sizes: 50 ug labelingCatalog #:
    92549
    , 92552, 92557, 92556, 92555, 92554, 92553, 92551, - 92550View allHide

    Label your antibody with one of our CF® Dyes specialized for STORM super-resolution microscopy. Labeling takes just 30 minutes without a purification step, and yields an antibody conjugate with a low degree of labeling optimal for STORM.

     
    Product

    DNP-dPEG4-NHS Ester

    From: $155 Sizes: 50 mg, 500 mgCatalog #:
    91062-500mg
    , - 91062-50mgView allHide

    A DNP moiety containing a PEG linker and an NHS ester terminal group which can be used for labeling biomolecules with a DNP hapten tag. The NHS ester will react with primary amines to form a stable amide bond while the hydrophilic PEG linker improves the solubility of the labeled bioconjugate.

     
    Product

    Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits

    From: $162 Sizes: 25 ug labeling, 100 ug labelingCatalog #:
    92561
    , 92560, 92559, 92558, 92563, 92562, 92565, 92564, 92567, 92566, 92569, 92568, 92571, 92570, 92573, 92572, 92575, - 92574View allHide

    Label your IgM antibody with one of our superior CF® Dyes in 15-30 minutes without a purification step.

     
    Product

    ROX Cadaverine, Trifluoroacetate Salt

    From: $162 Sizes: 5 mg, 10 mgCatalog #:
    90108
    , 91060, - 90121View allHide

    ROX cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides.

     
    Product

    6-TAMRA Cadaverine, Trifluoroacetate Salt

    From: $134 Sizes: 5 mgCatalog #: 90122

    6-Tetramethylrhodamine (TAMRA) cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides.

     
    Product

    5-(and-6)-Carboxyrhodamine 6G, Hydrochloride

    From: $117 Sizes: 5 mgCatalog #: 90120

    The free acid form of orange fluorescent 5-(and-6)-carboxyrhodamine 6G mixed isomers.

     
    Product

    Mix-n-Stain™ CF® Dye Antibody Labeling Kits

    From: $129 Sizes: 3x(5-20 ug) labelings, 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labelingCatalog #:
    92576
    , 92577, 92578, 92582, 92583, 92584, 92579, 92580, 92581, 92458, 92459, 92460, 92457, 92456, 92455, 92463, 92462, 92461, 92446, 92445, 92447, 92448, 92449, 92433, 92432, 92431, 92430, 92429, 92428, 92427, 92426, 92425, 92230, 92231, 92232, 92233, 92234, 92235, 92236, 92237, 92238, 92239, 92240, 92241, 92242, 92243, 92245, 92246, 92247, 92248, 92250, 92251, 92252, 92253, 92254, 92255, 92256, 92257, 92258, 92259, 92260, 92261, 92262, 92263, 92264, 92265, 92267, 92268, 92270, 92271, 92272, 92273, 92274, 92275, 92276, 92277, 92278, 92279, 92280, 92281, 92282, 92283, 92284, 92285, 92287, 92288, 92289, 92290, 92291, 92303, 92304, 92305, 92316, 92317, 92318, 92319, 92320, 92321, 92322, 92323, 92324, 92331, 92332, 92333, 92334, 92335, 92336, 92337, 92338, - 92339View allHide

    Label your IgG antibody with one of our superior CF® Dyes in as little as 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

     
    Product

    Tyramide Amplification Buffer Plus

    From: $131 Sizes: 250 reactions, 1000 reactionsCatalog #:
    22029
    , - 22029-TView allHide

    An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

     
    Product

    Ready-to-Use Tyramide Amplification Buffer, 1X

    From: $122 Sizes: 25 mL, 100 mLCatalog #:
    22027-25mL
    , - 22027-100mLView allHide

    For simple, optimized tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

     
    Product

    MB Acid

    From: $316 Sizes: 5 mgCatalog #: 40076

    MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.