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Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits

Label your antibody with one of our CF® Dyes specialized for STORM super-resolution microscopy. Labeling takes just 30 minutes without a purification step, and yields an antibody conjugate with a low degree of labeling optimal for STORM.

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50 ug labeling
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Product Description

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits allow you to label 50 ug of your antibody with one of Biotium’s STORM CF® Dyes to produce an antibody conjugate with a low 1-2.5 DOL (degree of labeling) optimal for super-resolution STORM (Stochastic Optical Reconstruction Microscopy). Labeling takes just 30 minutes, with minimal hands-on time and no purification.


  • Label 50 ug antibody
  • Choice of 8 CF® Dye colors ideal for STORM
  • CF®583R and CF®597R are novel green-excited STORM dyes
  • Labeling optimized to provide low 1-2.5 DOL
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification, 100% recovery

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive STORM CF® Dye
  • Mix-n-Stain™ Storage Buffer

Superior CF® Dyes for STORM Imaging

These kits feature a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits are designed to offer a low degree of labeling (DOL, or dye molecules per antibody molecule) of 1-2.5, which is reported to be optimal for STORM (1). Learn more about CF® Dyes for STORM or our other fluorescent reagents validated for super-resolution microscopy.

Discover More with Green-Excitable CF® Dyes Developed for Multi-Color STORM

High-quality STORM imaging relies heavily on bright fluorescent dyes with excellent photoswitching properties. While there are several commonly used red-excited dyes suitable for STORM imaging such as CF®647, CF®660C and CF®680, generating comparable dyes for other excitation sources to produce quality multi-color STORM images remains a challenge. Biotium scientists have met this challenge by developing novel chemical modifications for green-excited fluorescent dyes to offer optimal performance for STORM super-resolution imaging . This includes CF®583R and CF®597R which were developed specifically for STORM imaging and display exceptional performance for multi-color STORM (2).

Two-color (d)STORM imaging of a fixed COS-7 cell: microtubules were immunolabeled with CF®647 conjugate, and mitochondria were immunolabeled with CF®583R conjugate. (d)STORM was performed under standard conditions using a standard tris-based (d)STORM imaging buffer containing 100 mM cysteamine and an oxygen scavenger. CF®647 was first imaged with 647 nm excitation, and CF®583R was next imaged with 561 nm excitation. Images courtesy of Bowen Wang, Michael Xiong, and Professor Ke Xu, College of Chemistry, University of California, Berkeley.

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye. Select the links in the Dye Features column to learn more about each CF® Dye and potential applications.

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits

ConjugationEx/EmSizeCatalog No.Dye Features
CF®568562/584 nm50 ug92550CF®568 Features
CF®583R585/609 nm50 ug92551CF®583R Features
CF®594ST593/620 nm50 ug92552CF®594ST Features
CF®597R597/619 nm50 ug92553CF®597R Features
CF®647652/668 nm50 ug92554CF®647 Features
CF®660C667/685 nm50 ug92555CF®660C Features
CF®680681/698 nm50 ug92556CF®680 Features
CF®750755/779 nm50 ug92557CF®750 Features

Other Labeling Kits

We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

CF is a registered trademark of Biotium, Inc.


  1. Proc. SPIE 9331, (2015), DOI: 10.1117/12.2083209
  2. Angew. Chem. Int. Ed. 9331, e202113612, (2021), DOI: 10.1002/ANIE.202113612

Download a list of Mix-n-Stain™ references.
Download a list of CF® Dye references.


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