Amine-Reactive Dyes

5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer.

Amine-reactive form of 5-carboxyrhodamine 6G single isomer.

Amine-reactive form of 6-carboxyrhodamine 6G single isomer.

CBQCA (3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) reacts specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. CBQCA also reacts with hydrophilic peptides and amino sugars.

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

The amine-reactive quencher, CFQ520 succinimidyl ester, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

Cyanine NS succinimidyl esters are non-sulfonated cyanine dyes that can be used to label proteins, oligonucleotides and nucleic acids.

Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines. It has a quantum yield of 0.

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.

Amine-reactive form of 5-carboxyfluorescein single isomer.

6-FAM, SE (full name: 6-Carboxyfluorescein, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxyfluorescein single isomer.

5-FAM-PEO₈ SE (full name: 5-Carboxyfluorescein-PEO₈ propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group.

FITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines.

Fluorescamine is nonfluorescent until it reacts with primary amines. The reaction is completed in miniseconds and can be used to detect amine-containing compounds including peptides and proteins.

NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.