Biotinylation Reagents

Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

Aminooxy-biotin can be used to covalently attach biotin to aldehyde or ketone groups on polysaccharides, glycoproteins or antibodies. Aminooxy-biotin has also been used to efficiently label cell-surface glycans on living animal cells.

Biocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. Biocytin hydrazide can also be used to label glycoproteins or other carbohydrate molecules (See Reactive Dyes for Labeling Glycoproteins and Other Carbohydrate Molecules).

Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

Biotin cadaverine free base (N-(5-Aminopentyl)biotinamide) can be directly coupled to activated carboxylic acid groups without using an additional base.

Biotin cadaverine, biotin-X cadaverine (90061), and biotin ethylenediamine (90057) can be coupled to carboxylic acids, DNA and other biomolecules.

MTSEA biotin selectively labels thiols under mild conditions. It’s available in 3 variations: Biotin, Biotin-X, and Biotin-XX, all of which allow selective labeling of cell surface thiols.

Biotin NTA, also known as BNTA (Biotin nitrilotriacetic acid, tripotassium salt) is used to detect histidine-tagged proteins (as little as 0.1 pmol) immobilized on nitrocellulose membranes.

Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

Biotin SE (Biotin, succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA.