Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

MTS-TAMRA (MTS-5(6)-carboxytetramethylrhodamine, mixed isomers) is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye carboxytetramethylrhodamine to a thiol group.

MTSBS (4-Sulfonatobutyl methanethiosulfonate sodium salt) is a negatively charged MTS reagent.

MTSEA (2-Aminoethyl methanethiosulfonate hydrobromide) is a positively charged MTS reagent.

MTSEH (2-Hydroxyethyl methanethiosulfonate) is a neutral MTS reagent.

MTSES (2-Sulfonatoethyl methanethiosulfonate sodium salt) is a negatively charged MTS reagent.

MTSET (2-(Trimethylammonium)ethyl methanethiosulfonate, bromide) is a positively charged MTS reagent.

MTSPA (3-aminopropyl methanethiosulfonate hydrobromide) is a positively charged MTS reagent.

MTSPS (3-sulfonatopropyl methanethiosulfonate sodium salt) is a negatively charged MTS reagent.

MTSPT (3-trimethylammoniumpropyl methanethiosulfonate, bromide salt) is a positively charged MTS reagent.

N-(1,10-Phenanthrolin-5-yl)iodoacetamide has been used to label thiols to confer the metal-binding properties of the phenanthroline chelator. For example, completing of the chelator with copper can be used to selectively cleave RNA or DNA in the presence of hydrogen peroxide.

N-(6-Aminohexyl)maleimide is a cross-linking reagent that can react with an amine-reactive group and a thiol group.

NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

NBD fluoride (4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole) is similar to NBD chloride (90045) except that the former is much more reactive toward amines.

The fluorescence of NBD is highly dependent on the environment; it is virtually nonfluorescent in water but becomes strongly green fluorescent in hydrophobic media. NBD-ethylenediamine can be readily coupled to activated carboxy groups or sulfonyl chlorides.

NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond.

ROX cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides.