CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.
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Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
CF® Dye MTS
MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols.