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CPM

CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

Product Attributes

Excitation/Emission

384/469 nm (reaction product)

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Product Description

CPM (full name: 7-diethylamino-3-(4′-maleimidylphenyl)-4-methylcoumarin) is probably the most widely used blue fluorescent thiol-reactive dye. This maleimide derivative of coumarin is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. CPM is a good energy acceptor from tryptophan and a good energy donor to fluorescein. Please also see our MTS products.

  • Yellow solid soluble in anhydrous DMSO
  • MW: 402.45

 

References

1. Biochemistry 22, 5369 (1983).

2. Anal Lett 18, 393 (1985).

3. J Biol Chem 265, 14796 (1990).

 

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