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Can Mix-n-Stain Antibody Labeling Kits be used to label nanobodies?

We have had customers report successful labeling of nanobodies (single domain antibodies) with Mix-n-Stain Antibody Labeling Kits. Mix-n-Stain kits are optimized for labeling whole IgG (150 kDa) with 4-6 dyes per antibody. Due to their smaller size (~15 kDa), single chain antibodies have fewer potential labeling sites. So to increase the degree of labeling for single chain antibodies, we recommend using a higher ratio of dye to protein. For example, the Mix-n-Stain 50-100 ug size kit could be used to label 25-30 ug of nanobody. Also, we do not recommend using the 10K MWCO ultrafiltration vial with proteins smaller than 30 kDa. If ultrafiltration is required prior to Mix-n-Stain labeling, a 3K MWCO ultrafiltration vial (catalog no. 22018) should be used instead.

Category: Mix-n-Stain™ Antibody Labeling Kits

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