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Mix-n-Stain™ Nanobody Labeling Kits

These kits allow optimal labeling of single-chain nanobodies with CF® dyes or biotin in 30 minutes without a purification step.

Conjugation
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Size
Catalog #
price
Qty
5-20 ug labeling
20-50 ug labeling
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Product Description

Mix-n-Stain™ Nanobody Labeling Kits are the only kits specifically designed for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with fluorescent dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. The kit reduces the chance of over-labeling the nanobody, which can affect antigen binding. Labeling tolerates common buffer additives, including BSA.

The kits come with a selection of our next-generation fluorescent CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).

  • Label 5-20 ug or 20-50 ug of Nanobody®
  • Choice of 7 CF® Dye colors or biotin
  • Simple 30 minute labeling, no purification
  • Compatible with common stabilizers
  • Prevent over-labeling
  • Dye options for super-resolution and 2-photon imaging (learn more)

Choosing the Right Kit for Your Application

Before selecting a Mix-n-Stain™ Nanobody Labeling Kit, please download the Product Protocol to see our Kit Compatibility and Protocol Selection guidelines.

For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.

Mix-n-Stain™ Nanobody Labeling Kits

Label Ex/Em Labeling size
(ug Nanobody®)
Catalog number
Biotin N/A 5-20 ug 92500
20-50 ug 92501
CF®405S 404/431 nm 5-20 ug 92502
20-50 ug 92503
CF®488A 490/515 nm 5-20 ug 92504
20-50 ug 92505
CF®568 562/583 nm 5-20 ug 92506
20-50 ug 92507
CF®594 593/614 nm 5-20 ug 92508
20-50 ug 92509
CF®640R 642/662 nm 5-20 ug 92510
20-50 ug 92511
CF®647 650/665 nm 5-20 ug 92512
20-50 ug 92513
CF®680R 680/701 nm 5-20 ug 92514
20-50 ug 92515
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

Antibody & Protein Labeling Kits

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes ≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min. • Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG ~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits CF® Dyes
FITC
25 ug IgM
100 ug IgM
15-30 min. • Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits CF® Dyes 50 ug IgG ~ 30 min. • Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes 5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min. • Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes 0.1 umol
small ligand
~ 30 min. • Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging Near-IR CF® Dyes 3 x 1 mg IgG ~ 2 hours • Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours • Dyes, buffers, & spin vials for labeling + purification

Nanobody is a registered trademark of Ablynx, a Sanofi company. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of Cytiva. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.
 

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