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Mix-n-Stain™ Nanobody Labeling Kits

These kits allow optimal labeling of single-chain nanobodies with CF® dyes or biotin in 30 minutes without a purification step.

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Catalog #
5-20 ug labeling
20-50 ug labeling
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Product Description

Mix-n-Stain™ Nanobody Labeling Kits are the only kits specifically designed for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with fluorescent dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. The kit reduces the chance of over-labeling the nanobody, which can affect antigen binding. Labeling tolerates common buffer additives, including BSA.

The kits come with a selection of our next-generation fluorescent CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).

  • Label 5-20 ug or 20-50 ug of Nanobody®
  • Choice of 7 CF® Dye colors or biotin
  • Simple 30 minute labeling, no purification
  • Compatible with common stabilizers
  • Prevent over-labeling
  • Dye options for super-resolution and 2-photon imaging (learn more)

Choosing the Right Kit for Your Application

Before selecting a Mix-n-Stain™ Nanobody Labeling Kit, please download the Product Protocol to see our Kit Compatibility and Protocol Selection guidelines.

For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.

Mix-n-Stain™ Nanobody Labeling Kits

Label Ex/Em Labeling size
(ug nanobody)
Catalog number
Biotin N/A 5-20 ug 92500
20-50 ug 92501
CF®405S 404/431 nm 5-20 ug 92502
20-50 ug 92503
CF®488A 490/515 nm 5-20 ug 92504
20-50 ug 92505
CF®568 562/583 nm 5-20 ug 92506
20-50 ug 92507
CF®594 593/614 nm 5-20 ug 92508
20-50 ug 92509
CF®640R 642/662 nm 5-20 ug 92510
20-50 ug 92511
CF®647 650/665 nm 5-20 ug 92512
20-50 ug 92513
CF®680R 680/701 nm 5-20 ug 92514
20-50 ug 92515
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.
Nanobody is a registered trademark of Ablynx, a Sanofi company. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of Cytiva. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.

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