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Mix-n-Stain™ Nanobody Labeling Kits

These kits allow optimal labeling of single-chain nanobodies with CF® dyes or biotin in 30 minutes without a purification step. The labeling tolerates common buffer components including BSA.

Conjugation
Size
Catalog #
price
Qty
5-20 ug labeling
20-50 ug labeling
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Product Description

Mix-n-Stain™ Nanobody Labeling Kits are designed specifically for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with one of Biotium’s bright and photostable CF® dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. Labeling tolerates common buffer additives, including BSA.

The kits come with a selection of our next-generation CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).

Features

  • Label 5-20 ug or 20-50 ug of Nanobody®
  • Choice of 7 CF® Dye colors or biotin
  • Simple 30 minute labeling, no purification
  • Compatible with common stabilizers
  • Dye options for super-resolution and 2-photon imaging (learn more)

Choosing the Right Kit for Your Application

Before selecting a Mix-n-Stain™ Nanobody Labeling Kit, please download the Product Protocol to see our Kit Compatibility and Protocol Selection guidelines.

For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.

Mix-n-Stain™ Nanobody Labeling Kits

LabelEx/EmLabeling size
(ug nanobody)
Catalog number
BiotinN/A5-20 ug92500
20-50 ug92501
CF®405S404/431 nm5-20 ug92502
20-50 ug92503
CF®488A490/515 nm5-20 ug92504
20-50 ug92505
CF®568562/583 nm5-20 ug92506
20-50 ug92507
CF®594593/614 nm5-20 ug92508
20-50 ug92509
CF®640R642/662 nm5-20 ug92510
20-50 ug92511
CF®647650/665 nm5-20 ug92512
20-50 ug92513
CF®680R680/701 nm5-20 ug92514
20-50 ug92515
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

 

Mix-n-Stain™ Product Lines

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 30 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
Fluorescein (FITC)
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555 (Cy®3)
Cyanine 647 (Cy®5)
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
Dinitrophenol (DNP)
Mix-n-Stain™
Fluorescent Protein
& Tandem Dye
Antibody Labeling Kits
APC
R-PE
PerCP
Tandem Dyes
20-50 ug IgG
50-100 ug IgG
~ 4 hours• Fast, simple labeling

• Minimal hands-on time

• No purification
Mix-n-Stain™ Enzyme
Antibody Labeling Kits
HRP
AP
GOx
HRP:
10-20 ug IgG
25-50 ug IgG
50-100 ug IgG

AP or GOx:
25-50 ug IgG
50-100 ug IgG
~ 2-3 hours
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cy® Dyes
1 mg IgG~ 30 min.
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification
Note: Kits are not recommended for labeling IgM antibodies. Cy dye is a registered trademark of GE Healthcare.

 


Nanobody is a registered trademark of Ablynx, a Sanofi company. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of GE Healthcare. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.
 

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