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Do I have to use secondary antibodies raised in the same host species?

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As a rule, we recommend using secondary antibodies from the same host for multiplex staining (for example, combining Goat Anti-Rabbit with Goat Anti-Mouse, or Donkey Anti-Rabbit with Donkey Anti-Mouse). If we don’t currently offer the particular secondary antibody conjugate you wish to use, be sure to contact us, we may be able to perform a custom conjugation for you.

In our experience, cross-reactivity between donkey and goat host secondary antibodies is low, but this is not guaranteed to be the case for every lot of antibody. If you decide to combine secondary antibodies from different hosts, we recommend performing the appropriate controls to make sure the two secondary antibodies are not cross-reacting with each other.

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