Mix-n-Stain kits are optimized for labeling IgG antibodies, but can be used to label other proteins. Customers have reported successful labeling of nanobodies and single chain antibodies. Mix-n-Stain kits labeling conditions may cause denaturation of IgM antibodies. There are also published reports of Mix-n-Stain labeling of enzymes and lectins. Note that any conjugation method, including Mix-n-Stain, may affect the biological activity of proteins. Also, some free unreactive dye may remain after Mix-n-Stain labeling, which could interfere with live cell staining or trafficking studies using fluorescently labeled proteins. The ultrafiltration vial provided in the kit can be used to remove free dye after labeling if necessary.
Also see our CF Dye SE Protein Labeling Kits, which include everything you need to label and purify up to 3 mg protein. We also offer VivoBrite Antibody Labeling Kits for labeling antibodies or other proteins with near-infrared CF dyes for small animal in vivo imaging.← FAQs