While customers have reported success using TrueBlack® Lipofuscin Autofluorescence Quencher to quench RBC autofluorescence, during in-house research we’ve only ever seen partial quenching for RBCs. Experimental samples can vary quite a lot in the intensity of autofluorescence depending on age and processing, so the effectiveness of RBC quenching may be variable.
When we test TrueBlack® Lipofuscin Autofluorescence Quencher in an assay, we evaluate lipofuscin quenching in human brain sections, but our standard QC for the dye is measuring purity and OD of the solution, not tissue staining.
Alternative products that may be more effective at quenching RBCs or ECM would be TrueBlack® Plus (Cat. No. 23014) or EverBrite™ Hardset with TrueBlack® (Cat. No. 23017-23019) however, these products have not been empirically evaluated for this application.
View more FAQs