Support & Resources

+1

2,3-Diaminonaphthalene

2,3 Diaminonaphthalene is a fluorometric probe for nitrite. It reacts  to form the fluorescent dye 1 H-naphthotriazole.

Product Attributes

CAS number

771-97-1

Molecular weight

158

Excitation/Emission

365/415 (After Reaction)

Storage Conditions

Store at 2 to 8 °C, Protect from light, Desiccate

Molecular Formula: C10H10N2

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
100 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS

Product Description

2,3-Diaminonaphthalene reacts with nitrosonium, which is formed from nitrite at low pH, to form the fluorescent dye 1 H-naphthotriazole. This method can be used to detect 10 nM to 10 uM of nitrite (NO2-) and is compatible with 96-well format.

  • Fluorometric probe for nitrite
  • Quantify 10 nM to 10 uM of nitrite
  • Yellow orange solid soluble in DMSO

Molecular Structure:

References

  1. Luminescence 14, 283 (1999), DOI: 10.1002/(SICI)1522-7243(199911/12)14:6<283::AID-BIO572>3.0.CO;2-G
  2. Methods Enzymol. 268, 105 (1996), DOI: 10.1016/s0076-6879(96)68013-6
  3. Anal Biochem 214, 11 (1993), DOI: 10.1006/abio.1993.1449
 

You may also like…

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    Nitric Oxide Generation Kit

    Various nitric oxide generators for probing biochemical and physiological functions and for controllable NO generation in solution.

    NOS Inhibitor Kit

    A collection of popular inhibitors of the nitric oxide synthase isoforms, commonly used for studies of the biochemical and physiological functions of NO.

    Dihydroethidium (Hydroethidium)

    Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes.

    Griess Reagent Kit

    A chemical solution that is used for absorbance-based nitrite detection in biological samples such as cell lysates or tissue perfusates.

    Dihydrorhodamine 123

    Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.