5-(and-6)-Carboxy-2′,7′- dichlorofluorescein
5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.
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Product Description
5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment. Also see the membrane-permeant compound dichlorofluorescein diacetate (51016).
- pKa = 4.8
- ε= 90,000
- Orange solid soluble in DMSO and pH>6 buffers
- Store at room temperature and protect from light, especially in solution
- C21H10Cl2O7
- MW: 445
- [111843-78-8]
λExλEm = 504/529 nm
References
1. Biochemistry 26, 6330 (1987).
2. Biochim Biophys Acta 985, 75 (1989).
3. Anal Biochem 187, 109 (1990).
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5-(and 6)-CFDA
5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).
5-(and-6)-Carboxy-2′,7′- dichlorofluorescein
5-(and-6)-carboxy-2',7'-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.
CDCFDA
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2',7'-dichlorofluorescein (51015) by intracellular esterases.
5-Carboxy-2′,7′-dichlorofluorescein (single isomer)
This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.
5-CFDA (5-Carboxyfluorescein diacetate, single isomer)
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).