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5-(and 6)-CFDA

5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).

Product Attributes

CAS number

124387-19-5

Applications

pH indicators

Cell permeability

Membrane permeant

Cellular localization

Nucleus & cytoplasm

pKa

pKa 6.5

Indicator type

Ratiometric

Colors

Green

Excitation/Emission

495/519 nm (pH 9) (after hydrolysis)

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Product Description

5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see catalog number 51013).

  • Off-white solid soluble in DMSO
  • Store desiccated at 4°C
  • C25H16O9.
  • MW: 460
  • [124387-19-5]

References

1. J Cell Sci 98, 343(1991).

2. Nature 295, 524 (1982).

3. J Immunol Meth 100, 261 (1987).

4. J Immunol Meth 33, 33 (1980).

 

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