DiOC16(3) is a lipophilic cyanine dye with two C16 hydrocarbon chains. It can be optimally excited by the 488 nm argon laser line with green fluorescence emission (λEx/λEm = 484/501 nm).
You may also like…
RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.
The styryl dye RH237 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons.
The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414 (61016), but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not (see references 1,2).
DiO/DPA Membrane Potential Detection Kit
The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a "stationary" FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change.
Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.