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Di-4-ANEPPS

Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.

Product Attributes

CAS number

90134-00-2

For live or fixed cells

For live/intact cells

Potential dependence

Fast-response membrane potential dye

Colors

Far-red

Excitation/Emission

496/705 nm (see product description)

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5 mg
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Product Description

Di-4-ANEPPS is a fast-responding membrane potential dye (1). Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye. Di-4-ANEPPS is internalized in cells rapidly, so it is mainly used for short-term experiments. We also offer Di-8-ANEPPS (catalog number 61012), which is more lipophilic and better retained in the outer leaflet of the plasma membrane.

  • λExEm (MeOH) = 496/705 nm*
  • Orange red solid soluble in DMF, ethanol or DMSO
  • Store at 4°C and protect from light, especially in solution
  • C28H36N2O3S
  • MW: 481
  • [90134-00-2]

* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.

 

References

1. Nature 355, 349 (1992).

2. Biochim Biophys Acta 1068, 149 (1991).

3. PLoS ONE 8(10), e75678 (2013).

 

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