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Di-4-ANEPPS

Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.

Product Attributes

CAS number

90134-00-2

For live or fixed cells

For live/intact cells

Potential dependence

Fast-response membrane potential dye

Colors

Far-red

Excitation/Emission

496/705 nm (see product description)

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5 mg
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Product Description

Di-4-ANEPPS is a fast-responding membrane potential dye (1). Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye. Di-4-ANEPPS is internalized in cells rapidly, so it is mainly used for short-term experiments. We also offer Di-8-ANEPPS (catalog number 61012), which is more lipophilic and better retained in the outer leaflet of the plasma membrane.

  • λExEm (MeOH) = 496/705 nm*
  • Orange red solid soluble in DMF, ethanol or DMSO
  • Store at 4°C and protect from light, especially in solution
  • C28H36N2O3S
  • MW: 481
  • [90134-00-2]

* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.

 

References

  1. Nature, 355, 349(1992), DOI: 10.1038/355349a0
  2. Biochim Biophys Acta, 1068(2), 149(1991), DOI: 10.1016/0005-2736(91)90203-K
  3. PLoS ONE 8(10), e75678 (2013), DOI: 10.1371/journal.pone.0075678
  4. Am J Phys Heart, 0(0), (2021), DOI: 10.1152/ajpheart.00185.2021
 

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