Nerve Terminal Staining Kits
Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and accessories for washing away or quenching extracellular dye fluorescence.
Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and background reducing agents for washing away or quenching extracellular dye fluorescence. Kits are available with SynaptoGreen™ C4 (equivalent to FM®1-43), the fixable green nerve terminal dye AM1-43, or SynaptoRed™ C2 (equivalent to FM®4-64).
The kits are available with a choice of background reducing agents. ADVASEP-7 is a sulfonated cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen™ C4 to stain brain slices. SCAS (4-Sulfonato calixarene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps. Sulforhodamine 101 has been used as a quencher dye to reduce SynaptoGreen™ C4 fluorescence.
See the Neuronal Probes Flyer for detailed information.
Nerve Terminal Dyes and Kits
|SynaptoGreen™ Dyes |
(Ex/Em ~480/660 nm in membranes)
|70020... 70053||View full list of SynaptoGreen™ Dyes|
|SynaptoRed Dyes™ |
(Ex/Em ~510/750 nm in membranes)
|70021... 70050||View full list of SynaptoRed™ Dyes|
|ADVASEP-7||70029||Sulfonated cyclodextrin that aids in removal of free dye during washes|
|SCAS||70037||Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7|
|Sulforhodamine 101||80101||Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes|
|Nerve Terminal Staining Kits|
|Nerve Terminal Staining Kit I||70030||Includes SynaptoGreen™ C4 and ADVASEP-7|
|Nerve Terminal Staining Kit II (A)||70031||Includes AM1-43 and ADVASEP-7|
|Nerve Terminal Staining Kit II (B)||70031-1||Includes AM1-43 and SCAS|
|Nerve Terminal Staining Kit III||70032||Includes SynaptoGreen™ C4 and Sulforhodamine 101|
|Nerve Terminal Staining Kit V||70034||Includes SynaptoRed™ C2 and ADVASEP-7|
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.