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Nerve Terminal Staining Kits

Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and accessories for washing away or quenching extracellular dye fluorescence.

Product Attributes

Cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Colors

Green, Far-red

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Product Description

Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and background reducing agents for washing away or quenching extracellular dye fluorescence. Kits are available with SynaptoGreen™ C4 (equivalent to FM®1-43), the fixable green nerve terminal dye AM1-43, or SynaptoRed™ C2 (equivalent to FM®4-64). ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (equivalent to FM®1-43) to stain brain slices. SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps. Sulforhodamine 101 has been used as a quencher dye to reduce SynaptoGreen™ C4 fluorescence.

See the Neuronal Probes Flyer for detailed information.

Nerve Terminal Staining Kit Components

Nerve Terminal Staining KitNerve Terminal DyeBackground ReducerCatalog no.
Nerve Terminal Staining Kit ISynaptoGreen™ C4 (5 x 1 mg )ADVASEP-7 (250 mg)70030
Nerve Terminal Staining Kit II (A)AM1-43 (1 mg)ADVASEP-7 (100 mg)70031
Nerve Terminal Staining Kit II (B)AM1-43 (1 mg)SCAS (100 mg)70031-1
Nerve Terminal Staining Kit IIISynaptoGreen™ C4 (5 x 1 mg)Sulforhodamine 101 (100 mg)70032
Nerve Terminal Staining Kit IVSynaptoRed™ C2 (5 x 1 mg)ADVASEP-7 (250 mg)70034
FM is a registered trademark of Thermo Fisher Scientific.

References

n/a

 

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