ExoBrite™ EV Membrane Staining Kits
Fluorescent staining kits for bright, clean staining of EVs and exosomes. Suitable for detection by flow cytometry or microscopy.
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Product Description
ExoBrite™ EV Membrane Staining Kits were designed to overcome some of the challenges of exosome detection, particularly in flow cytometry. ExoBrite™ stains bind to molecules in the exosome membrane for bright, specific staining, with little to no background.
Features
- EV and exosome membrane stains
- Designed for detection by flow cytometry
- Bright signal and low background
- Stain purified or bead-bound exosomes
- Compatible with antibody co-staining
- Available in 4 colors
Kit Components
- ExoBrite™ EV Membrane Stain
- ExoBrite™ Reconstitution Solution
- 1 vial of stain makes 100 uL of 500X staining solution
Extracellular vesicles (EVs), including exosomes, are released from cells and are able to carry biological information between cells in different parts of the body. Therefore exosomes are increasingly studied for their potential use in drug delivery and medical diagnostic applications. Biotium developed ExoBrite™ EV Membrane Staining Kits for fluorescent labeling and detection of EVs and exosomes by flow cytometry. Other potential applications include fluorescence microscopy or other fluorescent exosome detection systems.
ExoBrite™ stains were designed to overcome some of the challenges of exosome detection, particularly in flow cytometry. While some dyes may form aggregates of a similar size as exosomes or EVs, thus confounding analysis, ExoBrite™ stains show little to no background, allowing stained exosomes to be accurately identified. ExoBrite™ stains bind to molecules in the exosome membrane for bright, specific staining. Unlike most other membrane dyes, ExoBrite™ stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound exosomes.
Exosomes are often detected by using fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, & CD81. ExoBrite™ EV Membrane Stains can be combined with antibody staining, for multi-parameter analysis. Biotium sells CD9 and CD81 monoclonal antibodies verified for exosome staining, available purified or conjugated to a large selection of bright and soluble CF® Dyes.
Less background and better coverage over other exosome/EV stains
ExoBrite™ EV Membrane Stains were designed to offer exceptional signal:noise and more complete coverage of purified and bead-bound exosomes. In the figure below, lipophilic dye DiO and plasma membrane stain CellMask™ demonstrate an unacceptable amount of dye aggregation in gated exosomes. Other exosome stains such as ExoFlow-ONE™ and ExoGlow™ have less dye aggregation but also less coverage of exosomes when compared to ExoBrite™ membrane stains.


Product | Ex/Em | Detection channels | Size | Catalog Number |
---|---|---|---|---|
ExoBrite™ 410/450 EV Membrane Staining Kit | 416/452 nm | Pacific Blue™ | 100 Labelings | 30111-T |
500 Labelings | 30111 | |||
ExoBrite™ 490/515 EV Membrane Staining Kit | 490/516 nm | FITC | 100 Labelings | 30112-T |
500 Labelings | 30112 | |||
ExoBrite™ 560/585 EV Membrane Staining Kit | 562/584 nm | PE, Cy®3 | 100 Labelings | 30113-T |
500 Labelings | 30113 | |||
ExoBrite™ 640/660 EV Membrane Staining Kit | 642/663 nm | APC | 100 Labelings | 30114-T |
500 Labelings | 30114 |
Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.
Learn about ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 exosome markers by flow cytometry and western blotting. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM EV Membrane Stains available in four CF® Dyes validated for STORM.