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Fluo-3, Pentasodium Salt

Fluo-3, pentasodium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Product Attributes

CAS number

134907-84-9

Cell permeability

Membrane impermeant

Indicator type

Non-ratiometric

Excitation/Emission

506/526 nm

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Product Description

Fluo-3, pentasodium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

  • Kd: 450 nM
  • λExEm (low or high [Ca2+]) = 506/526 nm
  • ε (506 nm) = 86,000 M-1cm-1
  • Orange red solid soluble in DMSO and water (pH >6)
  • Store at 4°C. Protect from light, especially when in solution
  • C36H25Cl2N5N2O13
  • MW: 879
  • [134907-84-9]


 
 
BAPTA-based ion indicators like Fluo-3 have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.

References

  1. Methods Cell Biol 99, 113, (2021), DOI: 10.1016/B978-0-12-374841-6.00005-0
 

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