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GAD1 / GAD67 Monoclonal Mouse Antibody (GAD1/2391)

This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2).

Product Attributes

Antibody number

2391

Reactivity (target)

GAD67, GAD1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

GAD1/2391

Isotype

IgG1, kappa

Molecular weight

67 kDa

Synonyms

67kDa glutamic acid decarboxylase; CPSQ1; DCE1; GAD67; GAD1; Glutamate decarboxylase 1; SCP

Human gene symbol

GAD1

Entrez gene ID

2571

SwissProt

Q99259

Unigene

420036

Immunogen

Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)

Cellular localization

Cytoplasmic

Species reactivity

Human

Applications

Immunohistology (formalin), Western, ELISA

Application notes

ELISA: For coating order antibody without BSA, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Western: 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

K-562 or HEK293 Cells. Pancreas

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Validated in protein array

Monospecific

Research areas

Neuroscience

Cell/tissue expression

Brain

Primary Antibody Formats:

  • Conjugates: 100 ug/mL  in PBS 0.1% BSA 0.05% azide
  • HRP conjugates: 100 ug/mL in PBS 0.05% BSA, no azide
  • Purified: 200 ug/mL in PBS 0.05% BSA 0.05% azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no azide
Conjugation
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Product Description

This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

References

Kaufman DL, Erlander MG, Clare-Salzler M, Atkinson MA, Maclaren NK, Tobin AJ. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus. J Clin Invest. 1992 Jan;89(1):283-92.

 

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