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GAD2 / GAD65 Monoclonal Mouse Antibody (GAD2/1960)

This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2).

Product Attributes

Antibody number

1960

Reactivity (target)

GAD65, GAD2

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

GAD2/1960

Isotype

IgG2b, kappa

Molecular weight

65 kDa

Synonyms

DCE2; GAD2; Glutamic Acid Decarboxylase 2 (Pancreas); Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65kDa); Glutamic Acid Decarboxylase 65 (GAD65)

Human gene symbol

GAD2

Entrez gene ID

2572

SwissProt

Q05329

Unigene

231829

Immunogen

Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)

Cellular localization

Cytoplasmic

Species reactivity

Human

Applications

Immunohistology (formalin), Western, ELISA

Application notes

ELISA: For coating order antibody without BSA, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Western: 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

Pancreas or Brain.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Validated in protein array

Monospecific

Research areas

Neuroscience

Cell/tissue expression

Brain

Primary Antibody Formats:

  • Conjugates: 100 ug/mL  in PBS 0.1% BSA 0.05% azide
  • HRP conjugates: 100 ug/mL in PBS 0.05% BSA, no azide
  • Purified: 200 ug/mL in PBS 0.05% BSA 0.05% azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no azide
Conjugation
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Product Description

This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

References

Kaufman DL., et al. J Clin Invest. 1992 Jan;89(1):283-92.

 

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