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Glo-Plate Blue LED Illuminator

The Glo-Plate™ Blue LED Illuminator is a multi-functional blue LED light box. It can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure™.

062917 GloPlate
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E90004

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Product Description

The Glo-Plate™ Blue LED Illuminator is a light-weight, multi-functional LED light box. The LEDs in the Glo-Plate™ Blue were designed for optimal photolysis of PMAxx™– or PMA-treated samples in viability PCR applications.

While our standard PMA-Lite™ LED photolysis device was designed for use with microcentrifuge tubes, the Glo-Plate™ Blue device allows for more flexible assay set-ups, including multi-well plates for high-throughput assays. The base of the Glo-Plate™ Blue chamber contains blue LED lights topped with a diffuser, allowing for even illumination across samples.

The Glo-Plate™ Blue LED Illuminator  is also an excellent light source for developing the staining of gels stained with the visible blue DNA gel stain DNAzure™. DNAzure™ is a novel, visible blue DNA gel stain with incredibly high sensitivity. After staining with DNAzure™ the DNA bands first appear colorless, but then become dark blue after developing with light exposure. The benefit to using DNAzure™ instead of fluorescent DNA dyes is that once the stain has developed, the DNA can be visualized by eye, without the need for imaging equipment. This is particularly advantageous for processes such as gel excision.

 

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Support & Faq

PMA and PMAxx™ for viability PCR

Biotium offers the PMA-Lite™ LED Photolysis Device for light-induced cross-linking of PMAxx™ and PMA to dsDNA. The PMA-Lite™ is designed for samples in microcentrifuge tubes.

For users that have samples in a different format (for example, 96-well plates or filters), we will soon be releasing a new light box that will allow for greater flexibility of assay format.

Commercial halogen lamps (>600 W) for home use have been employed for photoactivating PMA in some publications, though results have not been consistent due to inevitable variation in the set-up configurations. If you decide to use a halogen lamp, we recommend that you lay tubes on a block of ice set 20 cm from the light source, on a rocking platform to ensure continuous mixing. Set the lamp so that the light source is pointing directly downward onto the samples (up to 45° downward slant is OK). Expose samples to light for 5-15 min.

Category: PMA and PMAxx™ for viability PCR

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The LEDs in PMA-Lite have brightness of 600-800 millicandela (mcd). There are three LEDs next to each tube (one bottom, two side) and the wavelength is 465-475 nm.

Category: PMA and PMAxx™ for viability PCR

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Both PMA and PMAxx™ are both offered as 20 mM solutions in water. We also offer PMA as a solid, and customers often ask whether it needs to be dissolved in DMSO. While in some publications users have prepared their PMA stock solution in DMSO, this is not necessary. Unlike the older viability dye EMA, which is not soluble in water, PMA and PMAxx™ are very water soluble.

Category: PMA and PMAxx™ for viability PCR

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DNAzure™ visible DNA gel stain

For optimal staining, we recommend diluting the 100X DNAzure™ stock to 1X working solution fresh each time. However, if a 1X solution stored at 4C and protected from light, the gel stain should to be good up to two weeks.

Category: DNAzure™ visible DNA gel stain

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Yes, the 1X DNAzure™ staining solution can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).

Category: DNAzure™ visible DNA gel stain

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We recommend storing DNAzure™ at 4C. After storage at room temperature, we have seen some loss of dye stability.

Category: DNAzure™ visible DNA gel stain

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.