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HSP27 Recombinant Monoclonal Mouse Antibody (rHSPB1/774)

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Product Attributes

Antibody number

#2308

Antibody reactivity (target)

HSP27

Antibody type

Primary

Host species

Mouse

Clonality

Recombinant Monoclonal

Clone

rHSPB1/774

Isotype

IgG1, kappa

Molecular weight

27 kDa

Synonyms

Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Human gene symbol

HSPB1

Entrez gene ID

3315

SwissProt

P04792

Unigene

520973

Immunogen

Recombinant full-length human HSPB1 protein

Antibody target cellular localization

Nucleus & cytoplasm

Species reactivity

Chicken, Chimpanzee, Human, Monkey, Mouse, Rat, Sheep

Antibody application notes

For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

Positive control

~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Endocrinology, Heat shock proteins

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

MAb rHSPB1/774 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2308, Anti-HSP27 (rHSPB1/774) 

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980. |Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 199

 

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