Monoclonal anti HSP27 (HSPB1/774)

BNUB0774-0
BNUB0774-1
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Catalog #: BNUB0774
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Product Description

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced ?p29? and ?24K? protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Georgopoulos, C. and Welch, W.J. 1993. Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol. 9: 601-634

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

HSP27 (HSPB1/774)

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone

HSPB1/774

Molecular weight

27 kDa

Synonyms
Entrez gene ID

3315

SwissProt

P04792

Unigene

520973

Immunogen

Recombinant human HSPB1 protein

Species reactivity

Chicken, Chimpanzee, Human, Mouse, Rat, Sheep

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Predominantly cytoplasmic with some nuclear

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.25-0.5 ug/mL

Positive control

~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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