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Mix-n-Stain™ Nanobody Thiol Labeling Kits

Label 5-50 ug of VHH (also known as Nanobodies®) containing a single free cysteine with Biotium’s bright and photostable CF® Dyes, with no purification after labeling.

Conjugation
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Size
Catalog #
price
Qty
5-20 ug labeling
20-50 ug labeling
Clear selection
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Product Description

Mix-n-Stain™ Nanobody Thiol Labeling Kits are designed for labeling VHH (also known as Nanobody® or single-domain antibodies) that contain a single free cysteine. The labeling is done within 2 hours in ambient conditions, and requires minimal hands-on time and no purification.

Note: This kit is for labeling VHH via a single cysteine (1x Cys) only. Please see our Mix-n-Stain™ Nanobody Labeling Kits for standard labeling of VHH, or view our full selection of labeling kits for labeling IgG antibodies or other biomolecules.

Features

  • Label 5-20 ug or 20-50 ug VHH with single exposed cysteine residue
  • Choice of 7 CF® Dye colors
  • Simple 2 hour labeling, no purification
  • No purification, 100% recovery

Kit components

  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ Reaction Buffer, 5X
  • Mix-n-Stain™ Activating Agent
  • Mix-n-Stain™ Quenching Buffer, 10X
  • Mix-n-Stain™ Storage Buffer, 4X

Superior CF® Dyes

These kits feature a choice of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choosing the Right Kit for Your Application

Before selecting a Mix-n-Stain™ Nanobody Thiol Labeling Kit, please download the Product Protocol to see our Kit Compatibility guidelines.

For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.

Mix-n-Stain™ Nanobody Thiol Labeling Kits

ConjugationEx/EmLabeling size
(ug Nanobody®)
Catalog No.Dye Features
CF®488A490/516 nm5-20 ug92585CF®488A Features
20-50 ug92586
CF®568562/584 nm5-20 ug92587CF®568 Features
20-50 ug92588
CF®583R585/609 nm5-20 ug92589CF®583R Features
20-50 ug92590
CF®594593/615 nm5-20 ug92591CF®594 Features
20-50 ug92592
CF®647652/668 nm5-20 ug92593CF®647 Features
20-50 ug92594
CF®680681/698 nm5-20 ug92595CF®680 Features
20-50 ug92596
CF®740742/767 nm5-20 ug92597CF®740 Features
20-50 ug92598
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility guidelines.

Antibody & Protein Labeling Kits

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
FITC
25 ug IgM
100 ug IgM
15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Nanobody Thiol Labeling Kits
CF® Dyes~ 2 hours• Optimized for Nanobodies® with single exposed cysteine residue
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

Nanobody is a registered trademark of Ablynx, a Sanofi company. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of Cytiva. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.

References

 

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