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Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling

The simplicity and convenience of Mix-n-Stain™ labeling, for conjugation of 1 mg IgG with our CF® dyes or other dyes.

Product Attributes

Labeling scale

1 mg

Purification method

Purification not required after labeling

Conjugation
Size
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1 mg labeling
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Product Description

Features:

  • Rapid, simple, covalent antibody conjugation
  • No purification required after labeling
  • Reaction takes only 30 minutes, with minimal hands-on time
  • Wide selection of our next-gen CF® dyes and other dyes
  • One labeling reaction of 1 mg IgG per kit

Kit Components:

  • Reaction Buffer
  • Vial of pre-measured lyophilized dye
  • Quenching Buffer
Download the Product Information Sheet (PI) for detailed instructions.

Mix-n-Stain™ Maxi Antibody Labeling Kits contain everything you need to rapidly label 1 mg of IgG with one of our next-generation CF® dyes or other label. Simply mix your antibody with the reaction buffer and optimally formulated dye provided, followed by a 30 minute incubation and addition of the provided stop buffer. Any remaining free dye or other label is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. Note the labeling protocol and buffer requirements for the Maxi kits differ from our original micro-scale Mix-n-Stain™ kits, see the Reaction Compatibility Chart below.

After the reaction, the antibody will be labeled with an average of 4-6 dye molecules per antibody molecule. Mix-n-Stain™ labeling is covalent, so labeled antibodies can be used for multiplex staining without transfer of dyes/labels between antibodies.  These kits are optimized for a single labeling reaction with 1 mg of IgG; the labeling conditions may cause IgM antibodies to denature.

Our 1 mg scale Mix-n-Stain™ Maxi Kits are available with a selection of our bright and photostable CF® dyes, as well as Cyanine 555 or Cyanine 647 (structurally identical to Cy®3 or Cy®5), or fluorescein/FITC. See Ordering Information below.

Choose the right kit for your application

Mix-n-Stain™ kits are not designed to be used for multiple labeling reactions. For smaller-scale conjugation, see our original Mix‑n‑Stain™ CF® Dye Antibody Labeling Kits; original Mix-n-Stain™ kits also have greater tolerance for antibody buffer additives, and provide special protocols for labeling antibodies in buffer with BSA or in ascites fluid.

Mix-n-Stain™ kits can be used to label other proteins, however, the degree of labeling (DOL, or number of dye molecules per antibody) may not be optimal, and the kits are not designed to allow for DOL measurement after labeling. For performing reactions to optimize DOL for your antibody or application, we recommend using our CF® Dye SE Protein Labeling Kits. The kits include everything you need to perform controlled conjugation reactions and post-labeling purification, as well as detailed instructions and correction factors for determining DOL for CF® dyes.

We offer a variety of kits for labeling antibodies and other proteins:

Learn about all of our Mix-n-Stain™ and protein labeling options, or see our Frequently Asked Questions (FAQs) for more information.

Reaction Compatibility for Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling

ComponentCompatibility
Sodium azideCompatible
Glycerol ≤ 10%: Compatible
Glycerol > 10%:Compatible after ultrafiltration*
Tris ≤20 mMCompatible
Tris >20 mMCompatible after ultrafiltration*
GlycineCompatible after ultrafiltration*
BSA or gelatinNot compatible; purify antibody**
Serum, cell culture supernatant, or ascites fluidNot compatible; purify antibody**
* Ultrafiltration vials are sold separately (catalog no. 22004).
** Protocol/reagents for antibody purification not provided.

Mix-n-Stain™ Maxi Ordering Information

DyeEx/Em Catalog number
CF®350347/448 nm92420
CF®405S404/431 nm92421
CF®405M408/452 nm92404
CF®488A490/515 nm92405
CF®555555/565 nm92406
CF®568562/583 nm92407
CF®594593/614 nm92408
CF®633630/650 nm92409
CF®647650/665 nm92410
CF®680681/698 nm92422
CF®750755/777 nm92423
CF®770770/797 nm92424
Fluorescein/FITC494/518 nm92411
Cyanine 555 (equivalent to Cy®3)555/565 nm92415
Cyanine 647 (equivalent to Cy®5)650/665 nm92419
Cy dye is a registered trademark of GE Healthcare.

 

Cy dye is a registered trademark of GE Healthcare; SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.
 

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