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Mix-n-Stain™ FITC Antibody Labeling Kit

Label your antibody with fluorescein (FITC) just 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

Product Attributes

Excitation/Emission

494/518 nm

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Technology

Size
Catalog #
price
Qty
5-20 ug labeling
20-50 ug labeling
50-100 ug labeling
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Product Description

Mix-n-Stain™ FITC Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody with fluorescein (FITC) in just 15 minutes, with minimal hands-on time and no purification.

Features

  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Less than 30 seconds of hands-on time
  • 15 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive Fluorescein
  • Mix-n-Stain™ Storage Buffer
Overview of Mix-n-Stain Labeling Protocol
Overview of the Mix-n-Stain™ Antibody Labeling Kit workflow. Click to enlarge.

Unrivaled Convenience

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. The presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Choose the Right Labeling Kit for Your Antibody

This kit is for labeling an antibody with the traditional green fluorescent dye fluorescein (FITC). For a green dye with superior brightness, photostability, and insensitivity to pH, see our CF®488A Mix-n-Stain™ Antibody Labeling Kit. Learn more about our next-generation CF® Dyes.

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

We offer Mix-n-Stain™ Kits for labeling antibodies, nanobodies, and small ligands with a wide selection of fluorescent dyes, enzymes, and other labels. See our full selection of Mix-n-Stain™ Antibody Labeling Kits below.

Antibody & Protein Labeling Kits

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes ≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min. • Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG ~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits CF® Dyes
FITC
25 ug IgM
100 ug IgM
15-30 min. • Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits CF® Dyes 50 ug IgG ~ 30 min. • Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes 5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min. • Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes 0.1 umol
small ligand
~ 30 min. • Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging Near-IR CF® Dyes 3 x 1 mg IgG ~ 2 hours • Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours • Dyes, buffers, & spin vials for labeling + purification

References

Download a list of Mix-n-Stain™ references.

 

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